Cai Hugh Y, van Dreumel Tony, McEwen Beverly, Hornby Geoff, Bell-Rogers Patricia, McRaild Pat, Josephson Gaylan, Maxie Grant
Animal Health Laboratory, Laboratory Services Division, University of Guelph, PO Box 3612, Guelph, Ontario, Canada N1H 6R8.
J Vet Diagn Invest. 2007 Jan;19(1):91-5. doi: 10.1177/104063870701900115.
A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.
已验证一种聚合酶链反应（PCR）检测方法用于检测猪肺组织中的猪肺炎支原体。该检测方法的检测限为每克接种猪肺炎支原体的肺样本0.18个菌落形成单位。在现场验证中，通过猪肺炎支原体PCR和荧光抗体（FA）检测对来自220例病例的426头猪进行了猪肺炎支原体感染检测。总共，103头猪肺（24.2%）在PCR检测中呈阳性，69头猪肺（16.2%）在FA检测中呈阳性，其中62头猪在PCR和FA检测中均呈阳性。大多数PCR阳性但FA检测阴性的病例具有与猪肺炎支原体感染相符的病变。通过贝叶斯建模，确定PCR的诊断敏感性和特异性分别为97.3%和93.0%。
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