Qiu Gang, Rui Yapei, Li Kun, Huang Shucheng, Han Zhaoqing, Wang Xiaoqiang, Jiang Wenteng, Luo Houqiang, Lan Yanfang, Li Jiakui
Department of Veterinary Medicine, Xizang Agriculture and Animal Husbandry College, Nyingchi, Tibet, 860000, China.
Department of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Trop Anim Health Prod. 2017 Oct;49(7):1545-1551. doi: 10.1007/s11250-017-1365-x. Epub 2017 Jul 24.
Enzootic pneumonia (EP), often caused by Mycoplasma hyopneumoniae, occurs in Tibetan pigs between October and December in Western China. The aim of this study was to determine the prevalence of M. hyopneumoniae in Tibetan pig herds and also the prevalence of infection. M. hyopneumoniae was detected using enzyme-linked immunosorbent assay, polymerase chain reaction (PCR), and 16S rRNA sequencing. Twenty-nine inflammatory gross-lesions were observed in 155 lungs of slaughtered pigs. Invasion of focal lymphocytes was confirmed by paraffin sectioning and hematoxylin-eosin staining of lung sections. Bronchoalveolar lavage fluid (BALF) from slaughtered Tibetan pigs and nasal swabs from others were assayed using PCR. The prevalence of M. hyopneumoniae in Tibetan pig herds (via ELISA) was 20.48% (93/454) in 3 provinces (Sichuan, Tibet autonomous region, and Qinghai) between October and December of 2014. The difference in prevalence among animals in six different growing stages was statistically significant (P < 0.05). Anti-M. hyopneumoniae antibody was detected in breeding sows (45.83%; 22/48) and piglets (50%; 3/6). PCR and gel electrophoresis of BALF showed that 6.45% (10/155) of pigs were positive for M. hyopneumoniae. The presence of M. hyopneumoniae in serum was higher in piglets and breeding sows than in any other group. In conclusion, the results of this study showed that M. hyopneumoniae is prevalent among Tibetan pigs between October and December in Western China. To the authors' knowledge, this is the first investigation of M. hyopneumoniae prevalence in Tibetan pigs of Western China using serological tests, PCR, and 16S rRNA sequencing.
地方性肺炎（EP）通常由猪肺炎支原体引起，在中国西部，藏猪在10月至12月期间会发生这种疾病。本研究的目的是确定藏猪群中猪肺炎支原体的流行率以及感染率。采用酶联免疫吸附测定、聚合酶链反应（PCR）和16S rRNA测序检测猪肺炎支原体。在155头屠宰猪的肺脏中观察到29处炎性肉眼病变。通过肺组织切片的石蜡切片和苏木精-伊红染色证实有局灶性淋巴细胞浸润。对屠宰藏猪的支气管肺泡灌洗液（BALF）和其他猪的鼻拭子进行PCR检测。2014年10月至12月期间，在3个省份（四川、西藏自治区和青海）的藏猪群中，通过ELISA检测到猪肺炎支原体的流行率为20.48%（93/454）。六个不同生长阶段动物的流行率差异具有统计学意义（P < 0.05）。在繁殖母猪（45.83%；22/48）和仔猪（50%；3/6）中检测到抗猪肺炎支原体抗体。BALF的PCR和凝胶电泳显示，6.45%（10/155）的猪猪肺炎支原体呈阳性。仔猪和繁殖母猪血清中猪肺炎支原体的检出率高于其他任何组。总之，本研究结果表明，在中国西部，10月至12月期间猪肺炎支原体在藏猪中普遍存在。据作者所知，这是首次利用血清学检测、PCR和16S rRNA测序对中国西部藏猪中猪肺炎支原体的流行情况进行调查。
