Klausen Joan, Ekeroth Lars, Grøndahl-Hansen Jan, Andresen Lars Ole
National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
J Vet Diagn Invest. 2007 May;19(3):244-9. doi: 10.1177/104063870701900303.
Lipopolysaccharide (LPS) antigen was purified from Actinobacillus pleuropneumoniae serovar 7 by phenol-water extraction and fractionated on a, S-100 Sephacryl column. High molecular weight fractions of LPS purified from the S-100 column were pooled and used as antigen in an indirect serovar 7 ELISA. The ELISA was evaluated with sera from pigs experimentally infected with 11 different A. pleuropneumoniae serovars of biotype 1. Estimation of sensitivity and specificity of the A. pleuropneumoniae serovar 7 ELISA was performed using pig sera from herds naturally infected with A. pleuropneumoniae serovar 7 as well as sera from herds free of infection with A. pleuropneumoniae serovar 7. When compared to the complement fixation test (CFT) as a reference test, the ELISA showed much higher sensitivity and statistically equivalent specificity.
通过酚水提取法从胸膜肺炎放线杆菌血清型7中纯化脂多糖（LPS）抗原，并在α-S-100葡聚糖凝胶柱上进行分级分离。从S-100柱上纯化的LPS高分子量级分被合并，并用作间接血清型7 ELISA中的抗原。用来自实验感染生物型1的11种不同胸膜肺炎放线杆菌血清型的猪的血清对该ELISA进行评估。使用来自自然感染胸膜肺炎放线杆菌血清型7的猪群的血清以及来自未感染胸膜肺炎放线杆菌血清型7的猪群的血清来评估胸膜肺炎放线杆菌血清型7 ELISA的敏感性和特异性。与作为参考试验的补体结合试验（CFT）相比，该ELISA显示出更高的敏感性和统计学上相当的特异性。
