Teshima Kaho, Lee Jina, To Ho, Kamada Takashi, Tazumi Akihiro, Hirano Haruna, Maruyama Minoru, Ogawa Torata, Nagai Shinya, Turni Conny, Tsutsumi Nobuyuki
Nippon Institute for Biological Science, 9-2221-1, Shinmachi, Ome, Tokyo 198-0024, Japan.
Yamanashi Prefecture Eastern Livestock Hygiene Service Center, 1001-1 Karakashiwa, Ishiwa, Fuefuki, Yamanashi 406-0034, Japan.
J Vet Med Sci. 2017 Dec 22;79(12):1968-1972. doi: 10.1292/jvms.17-0374. Epub 2017 Oct 24.
An indirect enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide extract as antigen was evaluated for detection of antibodies to Actinobacillus pleuropneumoniae serovar 15. The serovar 15 ELISA had a higher sensitivity and specificity than latex agglutination test for 63 and 80 sera from pigs experimentally infected and not infected with A. pleuropneumoniae, respectively. When the serovar 15 ELISA was applied to 454 field sera, high rates of seropositivity were found in pigs from farms infected with A. pleuropneumoniae serovar 15, but not in those from farms free of A. pleuropneumoniae serovar 15. The results suggest that the serovar 15 ELISA may be useful for the serological surveillance of infection with A. pleuropneumoniae serovar 15.
评估了一种以脂多糖提取物为抗原的间接酶联免疫吸附测定（ELISA），用于检测猪胸膜肺炎放线杆菌血清型15的抗体。对于分别来自实验性感染和未感染猪胸膜肺炎放线杆菌的63份和80份猪血清，血清型15 ELISA的敏感性和特异性均高于乳胶凝集试验。当将血清型15 ELISA应用于454份现场血清时，在感染猪胸膜肺炎放线杆菌血清型15的猪场的猪中发现了高血清阳性率，而在未感染猪胸膜肺炎放线杆菌血清型15的猪场的猪中未发现。结果表明，血清型15 ELISA可能有助于猪胸膜肺炎放线杆菌血清型15感染的血清学监测。
