Hu Jiafen, Peng Xuwen, Budgeon Lynn R, Cladel Nancy M, Balogh Karla K, Christensen Neil D
Jake Gittlen Cancer Research Foundation, 500 University Dr., Pennsylvania State University, Hershey, PA 17033, USA.
J Virol. 2007 Jul;81(13):7171-7. doi: 10.1128/JVI.00200-07. Epub 2007 Apr 25.
Three transgenic rabbit lines that express a well-characterized human major histocompatibility complex class I (MHC-I) gene (HLA-A2.1) have been established. All three lines carry the HLA-A2.1 heavy chain and are able to pass the transgene to their offspring with both the outbred and the inbred EIII/JC genetic background. HLA-A2.1 colocalizes exclusively with rabbit MHC-I on the cell surfaces. These HLA-A2.1 transgenic rabbits demonstrated infection patterns similar to those found after cottontail rabbit papillomavirus (CRPV) challenge when compared with results in normal rabbits, although higher regression rates were found in HLA-A2.1 transgenic rabbits. Because the CRPV genome can accommodate significant modifications, the CRPV/HLA-A2.1 rabbit model has the potential to be used to screen HLA-A2.1-restricted immunogenic epitopes from human papillomaviruses in the context of in vivo papillomavirus infection.
已建立了三种表达特征明确的人类主要组织相容性复合体 I 类（MHC-I）基因（HLA-A2.1）的转基因兔系。所有这三个品系都携带 HLA-A2.1 重链，并且能够将转基因传递给具有远交和近交 EIII/JC 遗传背景的后代。HLA-A2.1 仅在细胞表面与兔 MHC-I 共定位。与正常兔的结果相比，这些 HLA-A2.1 转基因兔在受到棉尾兔乳头瘤病毒（CRPV）攻击后表现出的感染模式相似，尽管在 HLA-A2.1 转基因兔中发现了更高的消退率。由于 CRPV 基因组能够容纳显著的修饰，CRPV/HLA-A2.1 兔模型有潜力用于在体内乳头瘤病毒感染的背景下筛选来自人乳头瘤病毒的 HLA-A2.1 限制性免疫原性表位。
