Lv Fa Qin, Duan Yun You, Liu Xi, Cao Tie Sheng, Wang Wen, Yuan Li Jun
Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical University, Xi'an, China.
Exp Anim. 2007 Apr;56(2):111-7. doi: 10.1538/expanim.56.111.
To explore a method of establishing a rabbit model of superior vena cava obstruction (SVCO) by injecting VX2 tumor cell suspension transcutaneously under ultrasound guidance.
A suspension of VX2 tumor cells was prepared under sterile conditions. Fifteen adult healthy New Zealand White rabbits were enrolled in the experiment. Under ultrasound guidance, about 0.1 ml of the living tumor cell suspension was transcutaneously injected in front of the anterior wall of the right superior vena cava (SVC). The lumen, wall, blood flow of SVCs and adjacent tissues were examined with gray-scale and color Doppler ultrasonography, every 3 days starting from the 9th day after injection. Meanwhile, CT scanning and digital subtraction angiography (DSA) were also performed. The rabbits were dissected immediately after death and tissue samples were collected for pathologic examination.
Fourteen out of 15 rabbits developed tumors that were located close to SVCs and/or SVCs cavity, which was shown by ultrasonography. The diameters of the tumors were 80.7 +/- 4.3 mm. These tumors grew close to SVCs area and resulted in compression and infiltration of SVCs. CT scanning and DSA confirmed the establishment of the SVCO model. The achievement rate of the SVCO model was 93.3%. No rabbit died of complications.
A method of establishing a rabbit SVCO model by injecting VX2 tumor cell suspension under ultrasonographic guidance was established successfully, and it proved to be simple, effective and repeatable. The imaging characteristics of this model are in good accordance with those of SVCO in patients.
探讨在超声引导下经皮注射VX2肿瘤细胞悬液建立兔上腔静脉阻塞（SVCO）模型的方法。
在无菌条件下制备VX2肿瘤细胞悬液。15只成年健康新西兰白兔纳入实验。在超声引导下，于右头臂干前方经皮向上腔静脉（SVC）前壁注射约0.1ml活肿瘤细胞悬液。自注射后第9天起，每3天用灰阶及彩色多普勒超声检查SVC的管腔、管壁、血流情况及相邻组织。同时行CT扫描及数字减影血管造影（DSA）。兔死亡后立即解剖，取组织样本行病理检查。
15只兔中有14只超声显示肿瘤位于SVC附近和/或SVC腔内，肿瘤直径为80.7±4.3mm。这些肿瘤靠近SVC区域生长，导致SVC受压及浸润。CT扫描及DSA证实SVCO模型建立成功，SVCO模型成功率为93.3%。无兔死于并发症。
成功建立了在超声引导下经皮注射VX2肿瘤细胞悬液制备兔SVCO模型的方法，该方法简便、有效、可重复，模型的影像学特征与人类SVCO的特征相符。
