Gareau Daniel S, Merlino Glenn, Corless Christopher, Kulesz-Martin Molly, Jacques Steven L
Department of Dermatology, Oregon Health & Science University, Portland, Oregon 10022, USA.
J Invest Dermatol. 2007 Sep;127(9):2184-90. doi: 10.1038/sj.jid.5700829. Epub 2007 Apr 26.
A reflectance-mode confocal scanning laser microscope (rCSLM) was developed for imaging early-stage melanoma in a living mouse model without the addition of exogenous contrast agents. Lesions were first located by surveying the dorsum with a polarized light camera, then imaged with the rCSLM. The images demonstrated two characteristics of melanoma in this animal model: (1) melanocytes and apparent tumor nests in the epidermis at the stratum spinosum in a state of pagetoid spread and (2) architectural disruption of the dermal-epidermal junction. The epidermal melanocytes and apparent tumor nests had a high melanin content, which caused their reflectance to be fivefold greater than the surrounding epidermis. The rCSLM images illustrate the difference between normal skin and sites with apparent melanoma. This imaging modality shows promise to track the progression of melanoma lesions in animal models.
一种反射模式共聚焦扫描激光显微镜（rCSLM）被开发用于在不添加外源性造影剂的情况下对活体小鼠模型中的早期黑色素瘤进行成像。首先用偏振光相机对背部进行扫描以定位病变，然后用rCSLM进行成像。图像显示了该动物模型中黑色素瘤的两个特征：（1）棘层表皮中的黑素细胞和明显的肿瘤巢呈派杰样扩散状态；（2）真皮 - 表皮交界处的结构破坏。表皮黑素细胞和明显的肿瘤巢黑色素含量高，这导致它们的反射率比周围表皮高五倍。rCSLM图像说明了正常皮肤与明显黑色素瘤部位之间的差异。这种成像方式有望追踪动物模型中黑色素瘤病变的进展。
