Zhang Ying, Wang Wei, Lu Guo-Jun, Yu Wei-Ting, Guo Xin, Xiong Ying, Ma Xiao-Jun
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Sheng Wu Gong Cheng Xue Bao. 2007 Mar;23(2):303-9.
Microencapsulated recombinant cells technology is a novel approach to tumors therapy. It is necessary to prepare a plenty of the microcapsules with better cell viability and higher endostatin production in order to bring this technology into the clinic. The in vitro culture and cryopreservation are very important parameters in the preparation of microencapsulated cells. In this work, we studied the effect of the in vitro culture and cryopreservation on microencapsulated recombinant cells growth and endostatin production and the effect of the in vitro culture on the cryopreservation of microencapsulated recombinant cells. The results showed that the time of in vitro culture potently affected microencapsulated recombinant CHO cells growth in vivo, endostatin production and the microcapsule stability. The microcapsule kept intact after 36 days of implantation when the in vitro culture time was under 4 days. The thawed microencapsulated recombinant CHO cells had better cell growth and higher endostatin production after 40 days of cryopreservation when the in vitro culture time was 4 days and 8 days. Therefore, the best in vitro culture time was 4 days according to the results of the in vivo culture and cryopreservation and the cryopreservation did not affect microencapsulated recombinant CHO cells growth in vivo, endostatin production and the microcapsule stability.
微囊化重组细胞技术是一种新型肿瘤治疗方法。为使该技术应用于临床，制备大量具有较好细胞活力和较高内皮抑素产量的微囊是必要的。体外培养和冷冻保存是微囊化细胞制备过程中的重要参数。在本研究中，我们研究了体外培养和冷冻保存对微囊化重组细胞生长及内皮抑素产量的影响，以及体外培养对微囊化重组细胞冷冻保存的影响。结果表明，体外培养时间对微囊化重组CHO细胞的体内生长、内皮抑素产量及微囊稳定性有显著影响。当体外培养时间在4天以内时，微囊在植入36天后仍保持完整。当体外培养时间为4天和8天时，冷冻保存40天后解冻的微囊化重组CHO细胞具有更好的细胞生长和更高的内皮抑素产量。因此，根据体内培养和冷冻保存结果，最佳体外培养时间为4天，且冷冻保存不影响微囊化重组CHO细胞的体内生长、内皮抑素产量及微囊稳定性。
