肿瘤坏死因子-α上调免疫球蛋白分泌成分的表达。

Tumor necrosis factor-alpha upregulates the expression of immunoglobulin secretory component.

作者信息

Liu D Y, Wang X L, Liu P

机构信息

Clinical Hospital Department of Medical Examination, China Medical University No. 2, Shenyang, China.

出版信息

J Investig Allergol Clin Immunol. 2007;17(2):101-6.

PMID:17460948

Abstract

BACKGROUND

The immunoglobulin (Ig) secretory component (SC) is the extracellular component of the polymeric Ig receptor (plgR) that is responsible for the transcytosis of newly synthesized IgA. In addition, the SC seems to play important roles in regulating eosinophil functions and in enhancing local immune responses. SC expression in HT-29 has been shown to increase in response to interferon-gamma, interleukin (IL) 4 and IL-1, but whether tumor necrosis factor (TNF) alpha affects SC expression is disputed.

OBJECTIVE

Our aim was to study whether TNF-alpha can affect the expression of SC in Caco-2 cells.

METHODS

We used immunocytochemistry, enzyme-linked immunosorbent assay, Western blot, and quantitative real-time polymerase chain reaction to test SC-positive cells, free SC in culture supernatants, plgR mRNA, and protein expression of SC.

RESULTS

TNF-alpha dose-dependently increased SC-positive cells, free SC in culture supernatants, plgR mRNA, and protein expression of SC.

摘要

背景

免疫球蛋白（Ig）分泌成分（SC）是多聚Ig受体（plgR）的细胞外成分，负责新合成的IgA的转胞吞作用。此外，SC似乎在调节嗜酸性粒细胞功能和增强局部免疫反应中发挥重要作用。已表明HT-29中SC的表达会因γ干扰素、白细胞介素（IL）-4和IL-1而增加，但肿瘤坏死因子（TNF）α是否影响SC表达存在争议。