植物乳杆菌可抑制肿瘤坏死因子-α诱导的上皮屏障功能障碍和白细胞介素-8分泌。

Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.

作者信息

Ko Jae-Sung, Yang Hye-Ran, Chang Ju-Young, Seo Jeong-Kee

机构信息

Department of Pediatrics, Seoul National University College of Medicine, 28 Yongon-dong, Chongro-gu, Seoul 110-744, Korea.

出版信息

World J Gastroenterol. 2007 Apr 7;13(13):1962-5. doi: 10.3748/wjg.v13.i13.1962.

DOI:10.3748/wjg.v13.i13.1962

PMID:17461497

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4146973/

Abstract

AIM

To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells.

METHODS

Caco-2 cells were incubated with TNF-alpha alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IkappaB-alpha.

RESULTS

A TNF-alpha-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-alpha-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IkappaB-alpha in TNF-alpha-treated Caco-2 cells.

CONCLUSION

Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-alpha is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.

摘要

目的

确定植物乳杆菌是否能改变肿瘤坏死因子-α（TNF-α）对肠上皮细胞的有害作用。

方法

将Caco-2细胞单独与TNF-α孵育或在植物乳杆菌存在的情况下孵育。采用跨上皮电阻来测量上皮屏障功能。使用酶联免疫吸附测定法测量肠上皮细胞分泌的白细胞介素8（IL-8）。细胞裂解液蛋白用抗细胞外调节激酶（ERK）、抗磷酸化ERK和抗IκB-α进行免疫印迹分析。

结果

植物乳杆菌抑制了TNF-α诱导的跨上皮电阻降低。植物乳杆菌减少了TNF-α诱导的IL-8分泌。植物乳杆菌抑制了TNF-α处理的Caco-2细胞中ERK的激活和IκB-α的降解。

结论

植物乳杆菌抑制了TNF-α诱导的上皮屏障功能障碍和IL-8分泌。益生菌可能通过改变信号转导途径来维持上皮屏障功能并抑制炎症反应。

相似文献

Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.

World J Gastroenterol. 2007 Apr 7;13(13):1962-5. doi: 10.3748/wjg.v13.i13.1962.

Lactobacillus plantarum L9 but not Lactobacillus acidophilus LA reduces tumour necrosis factor induced bacterial translocation in Caco-2 cells.

Benef Microbes. 2017 May 30;8(3):497-505. doi: 10.3920/BM2016.0019. Epub 2017 Apr 26.

TNF-alpha sensitizes HT-29 colonic epithelial cells to intestinal lactobacilli.

Exp Biol Med (Maywood). 2002 Sep;227(8):665-70. doi: 10.1177/153537020222700817.

Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells.

Vet Immunol Immunopathol. 2016 Apr;172:55-63. doi: 10.1016/j.vetimm.2016.03.005. Epub 2016 Mar 5.

Probiotics and commensals reverse TNF-alpha- and IFN-gamma-induced dysfunction in human intestinal epithelial cells.

Gastroenterology. 2006 Mar;130(3):731-46. doi: 10.1053/j.gastro.2005.12.015.

ameliorates tumour necrosis factor-induced bacterial translocation in Caco-2 cells by regulation of TLR4 expression.

J Med Microbiol. 2018 Jul;67(7):982-991. doi: 10.1099/jmm.0.000762.

Effect of live Lactobacillus plantarum L2 on TNF-alpha-induced MCP-1 production in Caco-2 cells.

Int J Food Microbiol. 2010 Aug 15;142(1-2):237-41. doi: 10.1016/j.ijfoodmicro.2010.05.024. Epub 2010 Jun 4.

Salmosan, a β-galactomannan-rich product, in combination with Lactobacillus plantarum contributes to restore intestinal epithelial barrier function by modulation of cytokine production.

J Nutr Biochem. 2017 Mar;41:20-24. doi: 10.1016/j.jnutbio.2016.11.011. Epub 2016 Nov 23.

Surface-layer protein from Lactobacillus acidophilus NCFM attenuates tumor necrosis factor-α-induced intestinal barrier dysfunction and inflammation.

Int J Biol Macromol. 2019 Sep 1;136:27-34. doi: 10.1016/j.ijbiomac.2019.06.041. Epub 2019 Jun 8.

Lactobacillus rhamnosus GG attenuates interferon-{gamma} and tumour necrosis factor-alpha-induced barrier dysfunction and pro-inflammatory signalling.

Microbiology (Reading). 2010 Nov;156(Pt 11):3288-3297. doi: 10.1099/mic.0.040139-0. Epub 2010 Jul 23.

引用本文的文献

Effects of dietary Lactobacillus postbiotics and bacitracin on the modulation of mucosa-associated microbiota and pattern recognition receptors affecting immunocompetence of jejunal mucosa in pigs challenged with enterotoxigenic F18 Escherichia coli.

J Anim Sci Biotechnol. 2024 Oct 11;15(1):139. doi: 10.1186/s40104-024-01098-1.

The Anti-Inflammatory and Curative Exponent of Probiotics: A Comprehensive and Authentic Ingredient for the Sustained Functioning of Major Human Organs.

Nutrients. 2024 Feb 16;16(4):546. doi: 10.3390/nu16040546.

Effects of probiotics on pancreatic inflammation and intestinal integrity in mice with acute pancreatitis.

BMC Complement Med Ther. 2023 May 22;23(1):166. doi: 10.1186/s12906-023-03998-7.

Effects of short-chain fatty acids on intestinal function in an enteroid model of hypoxia.

Front Physiol. 2022 Dec 5;13:1056233. doi: 10.3389/fphys.2022.1056233. eCollection 2022.

Probiotics and their beneficial effects on alcohol-induced liver injury in a rat model: the role of fecal microbiota.

BMC Complement Med Ther. 2022 Jun 22;22(1):168. doi: 10.1186/s12906-022-03643-9.

Effects of Probiotics at the Interface of Metabolism and Immunity to Prevent Colorectal Cancer-Associated Gut Inflammation: A Systematic Network and Meta-Analysis With Molecular Docking Studies.

Front Microbiol. 2022 May 27;13:878297. doi: 10.3389/fmicb.2022.878297. eCollection 2022.

Postbiotic effects of Lactobacillus fermentate on intestinal health, mucosa-associated microbiota, and growth efficiency of nursery pigs challenged with F18+Escherichia coli.

J Anim Sci. 2022 Aug 1;100(8). doi: 10.1093/jas/skac210.

Effects of on growth performance, serum biochemical parameters, antioxidant capacity, and immune function in suckling calves.

Open Life Sci. 2020 Dec 31;15(1):1033-1041. doi: 10.1515/biol-2020-0106. eCollection 2020.

The Effects of Pro-, Pre-, and Synbiotics on Muscle Wasting, a Systematic Review-Gut Permeability as Potential Treatment Target.

Nutrients. 2021 Mar 29;13(4):1115. doi: 10.3390/nu13041115.

L. Tubers (Tiger Nuts) Protect Epithelial Barrier Function in Caco-2 Cells Infected by Enteritidis and Promote Growth.

Nutrients. 2020 Dec 28;13(1):71. doi: 10.3390/nu13010071.

本文引用的文献

Alive and dead Lactobacillus rhamnosus GG decrease tumor necrosis factor-alpha-induced interleukin-8 production in Caco-2 cells.

J Nutr. 2005 Jul;135(7):1752-6. doi: 10.1093/jn/135.7.1752.

Probiotics and prebiotics in gastrointestinal disorders.

Curr Opin Gastroenterol. 2004 Mar;20(2):146-55. doi: 10.1097/00001574-200403000-00017.

Role of probiotics in the modulation of intestinal infections and inflammation.

Curr Opin Gastroenterol. 2004 Jan;20(1):22-6. doi: 10.1097/00001574-200401000-00006.

Probiotics and gastrointestinal diseases.

J Intern Med. 2005 Jan;257(1):78-92. doi: 10.1111/j.1365-2796.2004.01410.x.

Increased epithelial uptake of protein antigens in the ileum of Crohn's disease mediated by tumour necrosis factor alpha.

Gut. 2004 Dec;53(12):1817-24. doi: 10.1136/gut.2004.041426.

Live Lactobacillus rhamnosus [corrected] is essential for the inhibitory effect on tumor necrosis factor alpha-induced interleukin-8 expression.

Infect Immun. 2004 Sep;72(9):5308-14. doi: 10.1128/IAI.72.9.5308-5314.2004.

DNA from probiotic bacteria modulates murine and human epithelial and immune function.

Gastroenterology. 2004 May;126(5):1358-73. doi: 10.1053/j.gastro.2004.02.003.

Functional modulation of enterocytes by gram-positive and gram-negative microorganisms.

Am J Physiol Gastrointest Liver Physiol. 2004 Apr;286(4):G613-26. doi: 10.1152/ajpgi.00341.2003.

TNF-alpha-induced increase in intestinal epithelial tight junction permeability requires NF-kappa B activation.

Am J Physiol Gastrointest Liver Physiol. 2004 Mar;286(3):G367-76. doi: 10.1152/ajpgi.00173.2003.

Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells.

World J Gastroenterol. 2004 Feb 1;10(3):455-7. doi: 10.3748/wjg.v10.i3.455.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

植物乳杆菌可抑制肿瘤坏死因子-α诱导的上皮屏障功能障碍和白细胞介素-8分泌。

Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.

作者信息

Ko Jae-Sung, Yang Hye-Ran, Chang Ju-Young, Seo Jeong-Kee

机构信息

Department of Pediatrics, Seoul National University College of Medicine, 28 Yongon-dong, Chongro-gu, Seoul 110-744, Korea.

出版信息

World J Gastroenterol. 2007 Apr 7;13(13):1962-5. doi: 10.3748/wjg.v13.i13.1962.

DOI:10.3748/wjg.v13.i13.1962

PMID:17461497

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4146973/

Abstract

AIM

To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells.

METHODS

RESULTS

CONCLUSION

摘要

目的

确定植物乳杆菌是否能改变肿瘤坏死因子-α（TNF-α）对肠上皮细胞的有害作用。

方法

结果

结论

植物乳杆菌抑制了TNF-α诱导的上皮屏障功能障碍和IL-8分泌。益生菌可能通过改变信号转导途径来维持上皮屏障功能并抑制炎症反应。

植物乳杆菌可抑制肿瘤坏死因子-α诱导的上皮屏障功能障碍和白细胞介素-8分泌。

Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.

作者信息

机构信息

出版信息

AIM

METHODS

RESULTS

CONCLUSION

目的

方法

结果

结论

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

植物乳杆菌可抑制肿瘤坏死因子-α诱导的上皮屏障功能障碍和白细胞介素-8分泌。

Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha.

作者信息

机构信息

出版信息

AIM

METHODS

RESULTS

CONCLUSION

目的

方法

结果

结论