益生菌抑制肿瘤坏死因子-α诱导的HT29细胞白细胞介素-8分泌。
Probiotics inhibit TNF-alpha-induced interleukin-8 secretion of HT29 cells.
作者信息
Bai Ai-Ping, Ouyang Qin, Zhang Wen, Wang Chun-Hui, Li Sheng-Fu
机构信息
Department of Gastroenterology, Huaxi Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
出版信息
World J Gastroenterol. 2004 Feb 1;10(3):455-7. doi: 10.3748/wjg.v10.i3.455.
Abstract
AIM
To study the effect of probiotics on interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines.
METHODS
Colonic adenocarcinoma HT29 cells were cultured and divided into four groups: control, TNF-alpha (group T in short), bifidobacterium (group B), lactobacillus (group L). B. Longum and L. bulgaricus were suspended in culture medium with a concentration of 1 x 10(8) cfu/ml and added into 24 wells respectively. One hour later TNF-alpha (10 ng/ml) was added into each well of groups T, B, L. The supernatants were collected and measured for IL-8 after 3 hours, nuclear factor-kappaB (NF-kappaB) p65 was also examined by Western blotting.
RESULTS
There was less interleukin-8 secretion in HT29 cells when preincubated with B. Longum or L. bulgaricus compared with group T. Less p65 appeared in nuclei in groups B and L compared with group T, as detected by Western blot.
CONCLUSION
Probiotics can suppress interleukin-8 secretion in intestinal epithelia when stimulated by proinflammatory cytokines, which is most likely mediated by NF-kappaB.
摘要
目的
研究益生菌对促炎细胞因子刺激下肠上皮细胞白细胞介素-8分泌的影响。
方法
培养结肠腺癌HT29细胞并分为四组：对照组、肿瘤坏死因子-α（简称T组）、双歧杆菌组（B组）、乳酸杆菌组（L组）。将长双歧杆菌和保加利亚乳杆菌分别以1×10⁸ cfu/ml的浓度悬浮于培养基中，加入24孔板。1小时后，向T组、B组、L组的每孔中加入肿瘤坏死因子-α（10 ng/ml）。3小时后收集上清液并检测白细胞介素-8，同时通过蛋白质免疫印迹法检测核因子-κB（NF-κB）p65。
结果
与T组相比，长双歧杆菌或保加利亚乳杆菌预孵育的HT29细胞中白细胞介素-8分泌较少。蛋白质免疫印迹法检测显示，与T组相比，B组和L组细胞核中出现的p65较少。
结论
益生菌可抑制促炎细胞因子刺激下肠上皮细胞白细胞介素-8的分泌，这很可能是由NF-κB介导的。
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