Pan Hong-Cheng, Liang Fu-Pei, Mao Chang-Jie, Zhu Jun-Jie, Chen Hong-Yuan
Key Lab of Analytical Chemistry for Life Science (MOE), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
J Phys Chem B. 2007 May 24;111(20):5767-72. doi: 10.1021/jp0703049. Epub 2007 Apr 27.
Highly luminescent zinc(II)-bis(8-hydroxyquinoline) (Znq(2)) complex nanorods have been synthesized via a sonochemical route from the microemulsion containing zinc acetate and 8-hydroxyquinoline. The scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the products were rod-like morphology with a diameter of about 200-450 nm and a length of about 1-3 microm. A possible mechanism for the formation of Znq(2) nanorods is proposed that the ultrasound wave might urge the collision and fusion of initial Znq(2) nuclei to form nanorods. The photoluminescence (PL) and resonance light scattering (RLS) of the products were also investigated. The Znq(2) nanorods were found to be sensitive to several proteins, such as human serum albumin (HSA), bovine serum albumin (BSA), bovine hemoglobin (Hb), and egg albumin (EA), displaying an increase in intensities of both PL and RLS. The protein-concentration dependence of the PL and RLS intensities can be well described as a Langmuir-type binding isotherm. This is the first report on the enhancement of PL and RLS intensities of Znq(2) nanorods by proteins. On the basis of enhanced PL and RLS intensities, the protein could be detected at the nanogram per milliliter level. The experimental results clearly showed that the Znq(2) nanorods were good protein probes for easy and highly sensitive detection.
通过声化学方法，由含有醋酸锌和8-羟基喹啉的微乳液合成了高发光性的锌（II）-双（8-羟基喹啉）（Znq(2)）复合纳米棒。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像显示，产物呈棒状形态，直径约200-450nm，长度约1-3微米。提出了一种Znq(2)纳米棒形成的可能机制，即超声波可能促使初始Znq(2)核碰撞和融合形成纳米棒。还研究了产物的光致发光（PL）和共振光散射（RLS）。发现Znq(2)纳米棒对几种蛋白质敏感，如人血清白蛋白（HSA）、牛血清白蛋白（BSA）、牛血红蛋白（Hb）和蛋清蛋白（EA），PL和RLS强度均增加。PL和RLS强度对蛋白质浓度的依赖性可以很好地用朗缪尔型结合等温线来描述。这是关于蛋白质增强Znq(2)纳米棒PL和RLS强度的首次报道。基于增强的PL和RLS强度，可以在纳克每毫升水平检测蛋白质。实验结果清楚地表明，Znq(2)纳米棒是用于简便且高灵敏度检测的良好蛋白质探针。
