自动化学发光免疫分析中血清甲状腺球蛋白与抗甲状腺球蛋白抗体之间的相互干扰。
Mutual interference between serum thyroglobulin and antithyroglobulin antibody in an automated chemiluminescent immunoassay.
作者信息
Gao Yunchao, Yuan Zhibin, Yu Yongli, Lu Hankui
机构信息
The Immunoassay Laboratory, Department of Nuclear Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, China.
出版信息
Clin Biochem. 2007 Jun;40(9-10):735-8. doi: 10.1016/j.clinbiochem.2007.03.010. Epub 2007 Mar 28.
OBJECTIVES
To investigate the analytical interference between serum Tg and TgAb.
DESIGN AND METHODS
Tg and TgAb were measured on an automated chemiluminescent immunoassay system in mixed sera from DTC patients and individual samples spiked with exogenous Tg.
RESULTS
Tg and TgAb recoveries in mixed patient samples were inversely correlated with expected TgAb or Tg concentrations, respectively. Impaired TgAb recoveries were also found in 50% (10/20) samples with high Tg in the exogenous recovery tests.
CONCLUSIONS
Mutual but not equal analytical interference between Tg and TgAb is present and concentration-dependent with interpatient variability.
目的
研究血清甲状腺球蛋白（Tg）和甲状腺球蛋白抗体（TgAb）之间的分析干扰。
设计与方法
在自动化学发光免疫分析系统上，对分化型甲状腺癌（DTC）患者的混合血清以及添加了外源性Tg的个体样本进行Tg和TgAb检测。
结果
混合患者样本中Tg和TgAb的回收率分别与预期的TgAb或Tg浓度呈负相关。在外源性回收率测试中，50%（10/20）的高Tg样本中也发现了TgAb回收率受损的情况。
结论
Tg和TgAb之间存在相互但不等同的分析干扰，且具有浓度依赖性和患者间变异性。
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