Arriagada Osvaldo, Constandil Luis, Hernández Alejandro, Barra Rafael, Soto-Moyano Rubén, Laurido Claudio
Laboratory of Neurobiology, Department of Biology Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago, Chile.
Int J Neurosci. 2007 May;117(5):617-25. doi: 10.1080/00207450600773806.
To investigate the contribution of glial cells in the spinal cord nociceptive transmission, the effect of intrathecally administered interleukin-1beta (IL-1beta) was studied in rats treated with the glial cell inactivator propentofylline and submitted to a C-fiber-mediated reflex paradigm evoked by single and repetitive (wind-up) electric stimulation. Intrathecal IL-1beta did not modify the C reflex integrated activity in either group of animals, while producing increased wind-up in intact and decreased wind-up in propentofylline pre-treated rats. Results suggest that the excitatory effect of IL-1beta on spinal wind-up activity in healthy rats is produced by a glial mediator, whereas the inhibitory effect resulted from a direct effect of the cytokine on dorsal horn neurons.
为研究脊髓胶质细胞在伤害性感受传递中的作用，在给予胶质细胞灭活剂丙戊茶碱处理的大鼠中，研究鞘内注射白细胞介素-1β（IL-1β）的效果，并使其接受由单次和重复（累加）电刺激诱发的C纤维介导的反射模式。鞘内注射IL-1β在两组动物中均未改变C反射的整合活动，而在未处理的大鼠中产生累加增强，在丙戊茶碱预处理的大鼠中产生累加减弱。结果表明，健康大鼠中IL-1β对脊髓累加活动的兴奋作用由一种胶质细胞介质产生，而抑制作用则是细胞因子对背角神经元直接作用的结果。
