Caporale A, Brescia A, Galati G, Castelli M, Saputo S, Terrenato I, Cucina A, Liverani A, Gasparrini M, Ciardi A, Scarpini M, Cosenza U M
Department of Surgery "P. Valdoni", Sant'Andrea II Medicine Faculty, University of Rome "La Sapienza", Rome, Italy.
Anticancer Res. 2007 Mar-Apr;27(2):985-9.
Local therapy with IL-2 may be very effective in the treatment of different forms of cancer. The aim of this study was to determine the effectiveness of IL-2 locoregional application in the treatment of colon cancer.
Twenty eight syngenic BDIX rats were utilized in this study. The rats were divided into two groups of fourteen animals: group T (treatment) and group C (control). All rats of both groups were injected, under the splenic capsule, with T 10(7) DHD/K2/ TRb neoplastic cells. Then, within and around the site of the previous inoculation, the T group was injected with 1 ml of glucosate solutions + 0.1% albumin (BSA) containing 2.5 x 10(6) IU of IL-2 ( Proleukin-Chiron), whereas the C group was injected with 1 ml of BSA alone. After three weeks, rats were sacrificed and the liver and spleen were removed. The following parameters were considered: volume and weight, neoplastic-non neoplastic tissue index of the spleen, mitotic index and vascular density of splenic and hepatic lesions.
All the studied parameters showed statistically significant differences in treated and untreated animals.
This study of a murine model demonstrated that IL-2 locoregional therapy may be effective in the treatment of colon cancer.
白细胞介素-2（IL-2）局部治疗可能对多种癌症治疗非常有效。本研究的目的是确定IL-2局部区域应用在结肠癌治疗中的有效性。
本研究使用了28只同基因BDIX大鼠。将大鼠分为两组，每组14只动物：T组（治疗组）和C组（对照组）。两组所有大鼠均在脾包膜下注射10(7)个DHD/K2/TRb肿瘤细胞。然后，在先前接种部位及其周围，T组注射1 ml含有2.5×10(6)国际单位IL-2（普罗金-奇龙公司生产）的葡糖酸盐溶液+0.1%白蛋白（牛血清白蛋白），而C组仅注射1 ml牛血清白蛋白。三周后，处死大鼠并取出肝脏和脾脏。考虑以下参数：体积和重量、脾脏肿瘤性与非肿瘤性组织指数、脾脏和肝脏病变的有丝分裂指数和血管密度。
所有研究参数在治疗组和未治疗组动物中均显示出统计学上的显著差异。
对小鼠模型的这项研究表明，IL-2局部区域治疗可能对结肠癌治疗有效。
