Murray P R, Sandrock M J
Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110.
Am J Hosp Pharm. 1991 Nov;48(11):2419-21.
The ability of a biphasic blood-culture system to detect microbial contamination of a total nutrient admixture (TNA) was studied. A TNA consisting of amino acids, dextrose, lipid emulsion, electrolytes, and trace elements was prepared under aseptic conditions. Septi-Chek blood-culture bottles were then injected with a TNA sample and with one of various dilutions of five bacterial or fungal suspensions, and an agar slide unit was attached to each bottle. One bottle was injected only with a TNA sample. The bottles were incubated and examined once daily. Each bottle was inverted immediately after inoculation and at each observation time to allow the broth to wash over the agar slide unit. Because the lipid contained in the TNA caused the broth in all bottles to become turbid, it was impossible to determine whether microbial growth was present in the broth. Microbial growth, however, became evident in 24-72 hours on all slide units except the one attached to the bottle that had been injected only with TNA. The Septi-Chek biphasic blood-culture system appears to be useful in evaluating the sterility of TNAs.
研究了双相血培养系统检测全营养混合液（TNA）微生物污染的能力。在无菌条件下制备了一种由氨基酸、葡萄糖、脂肪乳剂、电解质和微量元素组成的TNA。然后将Septi-Chek血培养瓶注入TNA样本以及五种细菌或真菌悬液的不同稀释液之一，并在每个瓶子上连接一个琼脂玻片单元。一个瓶子仅注入TNA样本。将瓶子进行培养并每天检查一次。接种后以及每次观察时，立即将每个瓶子倒置，以使肉汤冲洗琼脂玻片单元。由于TNA中含有的脂肪使所有瓶子中的肉汤变得浑浊，因此无法确定肉汤中是否存在微生物生长。然而，除了连接到仅注入TNA的瓶子上的玻片单元外，所有玻片单元在24至72小时内都出现了明显的微生物生长。Septi-Chek双相血培养系统似乎可用于评估TNA的无菌性。
