Ilic Dusko
StemLifeLine Inc., San Carlos, CA 94070, USA.
Regen Med. 2006 Jan;1(1):95-101. doi: 10.2217/17460751.1.1.95.
Among the major obstacles impeding successful derivation and continuous culture of human embryonic stem cells (hESC) for therapeutic purposes, are the presence of feeder cells and feeder-conditioned media of animal origin. The risk of contamination with xenopathogens makes hESC cultured in this way unsafe for future use in regenerative medicine. A holy grail for investigators in the field will be to establish and maintain new hESC lines in completely feeder-free and serum-free defined conditions. Recently, propagation of hESC has become possible, using mammalian- or human-derived extracellular matrix (ECM) and conditioned medium from feeder cells. In addition, providing a three-dimensional ECM environment can even support the derivation of new hESC. In this review, we examine recent advances in the use and development of substrates suitable for the derivation and maintenance of hESC, and our current understanding of the effects of a three-dimensional ECM milieu on cellular behavior.
在阻碍人类胚胎干细胞（hESC）成功诱导分化并进行持续治疗性培养的主要障碍中，动物源饲养层细胞和饲养层条件培养基的存在是其中之一。被异种病原体污染的风险使得以这种方式培养的hESC在未来用于再生医学时不安全。该领域研究人员的一个圣杯将是以完全无饲养层和无血清的特定条件建立并维持新的hESC系。最近，利用哺乳动物或人类来源的细胞外基质（ECM）和饲养层细胞的条件培养基，hESC的增殖已成为可能。此外，提供三维ECM环境甚至可以支持新hESC的诱导分化。在本综述中，我们研究了适用于hESC诱导分化和维持的底物的使用和开发方面的最新进展，以及我们目前对三维ECM环境对细胞行为影响的理解。
