Collins Catherine M, Olstad Kjetil, Sterud Erik, Jones Catherine S, Noble Leslie R, Mo Tor Atle, Cunningham Carey O
FRS Marine Laboratory, Molecular Genetics, Victoria Road, Torry, Aberdeen, Scotland, UK.
Fish Shellfish Immunol. 2007 Oct;23(4):793-807. doi: 10.1016/j.fsi.2007.03.001. Epub 2007 Mar 14.
Analysis of differential gene expression in salmon (Salmo salar) blood following infection with the monogenean parasite Gyrodactylus salaris, resulted in the isolation of a thymidylate kinase gene not previously described from fish and which showed similarity to an LPS-inducible thymidylate kinase gene isolated from mouse macrophages. This salmon TYKi-like gene may play a role in an innate generalised response to pathogen infection as it was upregulated in salmon following infection with the parasite, and also in response to injection with the immunostimulants LPS and Poly I:C, used to emulate bacterial and viral infections, respectively. The possible role of this gene in the biosynthesis of mitochondrial DNA in activated macrophages, in response to G. salaris infection is discussed.
对感染单殖吸虫寄生虫鲑三代虫后的鲑鱼（大西洋鲑）血液中的差异基因表达进行分析，结果分离出了一个此前未在鱼类中描述过的胸苷酸激酶基因，该基因与从小鼠巨噬细胞中分离出的脂多糖诱导型胸苷酸激酶基因具有相似性。这个鲑鱼类TYKi基因可能在对病原体感染的先天性全身反应中发挥作用，因为在感染寄生虫后的鲑鱼中它被上调，并且在分别用于模拟细菌和病毒感染的免疫刺激剂脂多糖和聚肌胞苷酸注射后也会上调。本文讨论了该基因在活化巨噬细胞中响应鲑三代虫感染时在线粒体DNA生物合成中的可能作用。
