Shang Shao-Bin, Li Yi-Fei, Guo Jun-Qing, Wang Zhong-Tian, Chen Qing-Xin, Shen Hui-Gang, Zhou Ji-Yong
Laboratory of Virology and Immunology, Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, PR China.
Res Vet Sci. 2008 Feb;84(1):150-7. doi: 10.1016/j.rvsc.2007.02.007. Epub 2007 Apr 30.
Porcine circovirus type 2 (PCV2) has been recently associated with a number of disease syndromes, especially postweaning multisystemic wasting disease (PMWS). Herein, an alternative indirect enzyme-linked immunosorbent assay (ELISA) for detection of PCV2 antibody was developed using nuclear localization signal-truncated capsid protein of PCV2 produced in Escherichia coli (CAP ELISA). This assay was validated by comparison with an indirect immunofluorescence assay (IIF) and a PCV2-based ELISA. The diagnostic sensitivity (DSN), specificity (DSP) and accuracy of the CAP ELISA were 95.3%, 93.9% and 95.1%, compared with IIF on 1080 field serum samples, and 93.3%, 84.2% and 91.1%, compared with the PCV2-based ELISA on 79 field sera, respectively. Cross-reactivity assay showed that this assay was PCV2-specific. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were less than 15%. This ELISA is simpler to produce and perform, time-saving and suitable for large scale surveys of PCV2 infection at low cost and the evaluation of the efficiency of various vaccines against PCV2.
猪圆环病毒2型（PCV2）最近与多种疾病综合征相关，尤其是断奶后多系统消耗性疾病（PMWS）。在此，利用在大肠杆菌中产生的PCV2核定位信号截短的衣壳蛋白开发了一种用于检测PCV2抗体的替代间接酶联免疫吸附测定（ELISA）（CAP ELISA）。通过与间接免疫荧光测定（IIF）和基于PCV2的ELISA进行比较，对该测定进行了验证。在1080份田间血清样本上，与IIF相比，CAP ELISA的诊断敏感性（DSN）、特异性（DSP）和准确性分别为95.3%、93.9%和95.1%；在79份田间血清上，与基于PCV2的ELISA相比，分别为93.3%、84.2%和91.1%。交叉反应性测定表明该测定具有PCV2特异性。重复性试验显示，批次内和批次间阳性血清的变异系数均小于15%。这种ELISA生产和操作更简单，节省时间，适合以低成本对PCV2感染进行大规模调查以及评估各种抗PCV2疫苗的效果。
