Dogru Mehmet, Gul-Guven Reyhan, Erdogan Sait
Dicle University, Faculty of Science, Chemistry Department, 21280 Diyarbakir, Turkey.
J Hazard Mater. 2007 Oct 1;149(1):166-73. doi: 10.1016/j.jhazmat.2007.03.066. Epub 2007 Mar 25.
The present work proposes the use of Bacillus subtilis immobilized on Amberlite XAD-4 as new biosorbent in trace metal determination. The procedure is based on the biosorption of Cu and Cd ions on a column of Amberlite XAD-4 resin loaded with dried, dead bacterial components prior to their determination by flame AAS. Various parameters such as pH, amount of adsorbent, eluent type and volume, flow rate of solution and matrix interference effect on the retention of the metal ions have been studied. The optimum pH values of quantitative sorption for Cu and Cd were found to be 7.0 and 7.5, respectively. These metal ions can be desorbed with 1M HCl (recovery, 96-100%). The sorption capacity of the resin was 0.0297 and 0.035 mmol g(-1) for Cu(2+) and Cd(2+), respectively. The tolerance limit of some electrolytes were also studied. This procedure was applied to Cu(2+) and Cd(2+) determination in aqueous solutions, including river and well water systems. In order to evaluate the accuracy of the proposed procedure, the certified reference materials, NRCC-SLRS-4 Riverine water and LGC7162 Strawberry leaves were analyzed.
本研究提出将固定在Amberlite XAD - 4上的枯草芽孢杆菌用作痕量金属测定中的新型生物吸附剂。该方法基于在通过火焰原子吸收光谱法（flame AAS）测定之前，将铜离子和镉离子生物吸附在负载有干燥、死亡细菌成分的Amberlite XAD - 4树脂柱上。研究了各种参数，如pH值、吸附剂用量、洗脱液类型和体积、溶液流速以及基质干扰对金属离子保留的影响。发现铜和镉定量吸附的最佳pH值分别为7.0和7.5。这些金属离子可用1M盐酸解吸（回收率为96 - 100%）。该树脂对Cu(2+)和Cd(2+)的吸附容量分别为0.0297和0.035 mmol g(-1)。还研究了一些电解质的耐受极限。该方法应用于水溶液中Cu(2+)和Cd(2+)的测定，包括河水和井水系统。为了评估所提出方法的准确性，对认证参考物质NRCC - SLRS - 4河流水和LGC7162草莓叶进行了分析。
