Yildiz Technical University, Faculty of Art and Science, Department of Chemistry, Davutpasa Cad., No: 127, 34210-Davutpasa, Istanbul, Turkey.
Bioresour Technol. 2011 Sep;102(17):8035-9. doi: 10.1016/j.biortech.2011.05.004. Epub 2011 May 7.
The present work proposes the use of Pleurotus ostreatus immobilized on Amberlite XAD-4 as new biosorbent in trace metal determination. The effects of experimental parameters, such as "pH and flow rate of sample solution, amount of solid phase, eluent type, and concentration" on the recovery of the metal ions were investigated. Maximum adsorption of Cr(III), Cd(II) and Cu(II) ions took place in the pH range 4-5. These metal ions can be desorbed with 1M HCl (recovery 95-100%). 0.2g adsorbent amount and 2.5 mL min(-1) flow rate was found to be optimum of all preconcentration experiments. The sorption capacity after 10 cycles of sorption and desorption does not vary more than 2.0%. The influences of the contaminant ions on the retentions of the analytes were also examined. The results showed that P. ostreatus immobilized on Amberlite XAD-4 can be considered as very promising material in trace metal determination.
本工作提出了将固定在 Amberlite XAD-4 上的平菇（Pleurotus ostreatus）用作痕量金属测定中的新型生物吸附剂。考察了实验参数，如“样品溶液的 pH 值和流速、固相量、洗脱剂类型和浓度”对金属离子回收率的影响。Cr(III)、Cd(II)和 Cu(II)离子的最大吸附发生在 pH 值为 4-5 的范围内。这些金属离子可以用 1M HCl 洗脱（回收率为 95-100%）。在所有预浓缩实验中，发现 0.2g 吸附剂用量和 2.5mL min(-1)流速是最佳的。吸附和解吸 10 次后，吸附容量的变化不超过 2.0%。还考察了杂质离子对分析物保留的影响。结果表明，固定在 Amberlite XAD-4 上的平菇可以被认为是痕量金属测定中很有前途的材料。
