Dzidziul Igor, Gutowska Izabela, Noceń Iwona, Chlubek Dariusz
Zakład Biochemii, Katedra Biochemii i Chemii Medycznej Pomorskiej Akademii Medycznej al. Powstańców Wlkp. 72, 70-111 Szczecin.
Ann Acad Med Stetin. 2006;52 Suppl 1:17-20.
Enamel exhibits the highest degree of mineralization and is the hardest among tissues. Its non-organic material content is 96-98%, out of which 90% is in the form of hydroxyapatite crystals. Exchange of ions is an important property of hydroxyapatite with the hydroxyl ion (OH-) exchanging particularly easily for the fluoride ion (F-). F- ions are capable of stabilizing the structure of hydroxyapatite by reducing its solubility and in consequence increasing enamel resistance to caries. The aim of this work was to compare the content of fluoride in superficial layers of enamel obtained from permanent and deciduous teeth.
We collected 55 human permanent and 11 deciduous teeth without any evident damage to or discoloration of the enamel. Microsamples of enamel from the paragingival part on the buccal surface of the tooth were obtained using acid biopsy. Fluoride content was determined using an ion-selective electrode. Calcium content was measured with atomic absorption spectrometry.
We found that enamel of deciduous teeth is more susceptible to acid digestion than in the case of permanent teeth, although the difference was not statistically significant. The content of fluoride decreased with increasing depth of digestion. Deciduous teeth contained significantly less fluoride in every layer studied in comparison with permanent teeth.
牙釉质呈现出最高程度的矿化，是所有组织中最坚硬的。其无机物质含量为96 - 98%，其中90%以羟基磷灰石晶体的形式存在。离子交换是羟基磷灰石的一项重要特性，其中氢氧根离子（OH-）特别容易与氟离子（F-）发生交换。氟离子能够通过降低羟基磷灰石的溶解度来稳定其结构，从而提高牙釉质的抗龋能力。本研究的目的是比较恒牙和乳牙牙釉质表层的氟含量。
我们收集了55颗恒牙和11颗乳牙，这些牙齿的牙釉质没有任何明显损伤或变色。使用酸蚀活检法从牙齿颊面牙龈旁部分获取牙釉质微样本。使用离子选择性电极测定氟含量。用原子吸收光谱法测量钙含量（注：原文中此句“Calcium content was measured with atomic absorption spectrometry.”在翻译时按照中文表达习惯调整了语序，更符合中文逻辑）。
我们发现乳牙牙釉质比恒牙牙釉质更容易被酸消解，尽管差异无统计学意义。氟含量随着消解深度的增加而降低。与恒牙相比，乳牙在每个研究层中的氟含量都显著更低。
