Wahlquist S P, Eberhard M L
Division of Parasitic Diseases, Atlanta, Georgia 30333.
Ann Trop Med Parasitol. 1991 Jun;85(3):365-8. doi: 10.1080/00034983.1991.11812575.
Sodium azide was compared with 10% formalin to evaluate sodium azide's effectiveness as a faecal preservative for intestinal helminths and protozoa. Faecal specimens collected from Haiti were preserved in sodium azide and in 10% formalin and analysed after 1.5, 6.5 and 11.5 weeks by examining direct wet-mount preparations. Sodium azide did not preserve the morphology of either helminths or protozoa as well as 10% formalin did. However, sodium azide prevented embryogenesis of helminth eggs, while some helminth eggs in 10% formalin contained living larvae. Biosafety guidelines regarding the toxicity, reactivity, and disposal of sodium azide were strictly followed. Use of 10% formalin is a significantly better choice than sodium azide for preserving parasites when accurate identification of parasites and biosafety are the main concerns.
将叠氮化钠与10%甲醛进行比较,以评估叠氮化钠作为肠道蠕虫和原生动物粪便防腐剂的有效性。从海地收集的粪便标本分别保存在叠氮化钠和10%甲醛中,并在1.5、6.5和11.5周后通过检查直接湿片制备物进行分析。叠氮化钠对蠕虫或原生动物形态的保存效果不如10%甲醛。然而,叠氮化钠可阻止蠕虫卵的胚胎发育,而10%甲醛中的一些蠕虫卵含有活幼虫。严格遵循了关于叠氮化钠毒性、反应性和处置的生物安全指南。当主要关注寄生虫的准确鉴定和生物安全时,使用10%甲醛作为保存寄生虫的选择明显优于叠氮化钠。