Ko Jae-hyeong, Altman Sidney
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Proc Natl Acad Sci U S A. 2007 May 8;104(19):7815-20. doi: 10.1073/pnas.0701715104. Epub 2007 Apr 30.
OLE (ornate, large, and extremophilic) RNA is a noncoding RNA that is found in several extremophilic bacteria, including Bacillus halodurans. The function of OLE RNA has not been clarified. In this study, we found that RNase P cleaves OLE RNA and that the cleavage leads to a small reduction of expression of a downstream gene determined by analyses in vitro and in vivo. Under RNase P-deficient conditions, the amount of OLE RNA increased. Our results imply that RNase P could play a role in the regulation of gene expression in relation to conserved RNA motifs like OLE RNA as well as in riboswitches and operons.
OLE(华丽、大型且嗜极端环境的)RNA是一种非编码RNA,存在于几种嗜极端环境细菌中,包括嗜碱芽孢杆菌。OLE RNA的功能尚未明确。在本研究中,我们发现核糖核酸酶P可切割OLE RNA,且这种切割会导致下游基因表达略有降低,这是通过体外和体内分析确定的。在缺乏核糖核酸酶P的条件下,OLE RNA的量增加。我们的结果表明,核糖核酸酶P可能在与OLE RNA等保守RNA基序相关的基因表达调控中发挥作用,以及在核糖开关和操纵子中发挥作用。