Li Yong, Altman Sidney
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13213-8. doi: 10.1073/pnas.2235589100. Epub 2003 Oct 29.
The rnpA mutation, A49, in Escherichia coli reduces the level of RNase P at 43 degrees C because of a temperature-sensitive mutation in C5 protein, the protein subunit of the enzyme. Microarray analysis reveals the expression of several noncoding intergenic regions that are increased at 43 degrees C compared with 30 degrees C. These regions are substrates for RNase P, and they are cleaved less efficiently than, for example, tRNA precursors. An analysis of the tna, secG, rbs, and his operons, all of which contain RNase P cleavage sites, indicates that RNase P affects gene expression for regions downstream of its cleavage sites.
大肠杆菌中的rnpA突变A49,由于该酶的蛋白质亚基C5蛋白发生温度敏感突变,在43℃时会降低核糖核酸酶P的水平。微阵列分析显示,与30℃相比,几个非编码基因间区域在43℃时表达增加。这些区域是核糖核酸酶P的底物,并且它们的切割效率低于例如tRNA前体。对tna、secG、rbs和his操纵子的分析表明,所有这些操纵子都含有核糖核酸酶P切割位点,这表明核糖核酸酶P会影响其切割位点下游区域的基因表达。