Behrendt Undine, Ulrich Andreas, Schumann Peter, Meyer Jean-Marie, Spröer Cathrin
Leibniz-Centre for Agricultural Landscape Research (ZALF), Institute of Landscape Matter Dynamics, Eberswalder Str. 84, D-15374 Müncheberg, Germany.
DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2007 May;57(Pt 5):979-985. doi: 10.1099/ijs.0.64793-0.
The taxonomic position of a group of fluorescent pseudomonad strains isolated from the phyllosphere of grasses was investigated through a polyphasic approach. Riboprinting analysis revealed highly similar patterns for the investigated strains which supported, together with the agreement of many phenotypic characteristics, their affiliation to the same species. A comparison of 16S rRNA gene sequences of strain P 513/18(T), a representative strain from the grass isolates, revealed that it was affiliated to the cluster of the 'Pseudomonas fluorescens group', with Pseudomonas costantinii as the closest phylogenetic neighbour. However, DNA-DNA hybridization showed a clear demarcation at the species level between strain P 513/18(T) and P. costantinii. Furthermore, a comparison of riboprint patterns with Pseudomonas species clustering next to the novel grass isolates on the basis of 16S rRNA gene sequences supported their separate species status at the phylogenetic level. Based on phenotypic features, the novel isolates could also be differentiated from the other fluorescent Pseudomonas species that share positive arginine dihydrolase and oxidase reactions. As a consequence of these phenotypic and phylogenetic analyses, the isolates from the grass pyllosphere represent a novel species for which the name Pseudomonas lurida sp. nov. is proposed. The type strain is P 513/18(T) (=DSM 15835(T)=LMG 21995(T)).
通过多相分类法研究了从禾本科植物叶际分离出的一组荧光假单胞菌菌株的分类地位。核糖体印记分析显示，所研究菌株的图谱高度相似，这与许多表型特征的一致性共同支持了它们属于同一物种。对禾本科植物分离株的代表菌株P 513/18(T)的16S rRNA基因序列进行比较，结果表明它属于“荧光假单胞菌群”，与康氏假单胞菌（Pseudomonas costantinii）是最接近的系统发育邻种。然而，DNA-DNA杂交显示菌株P 513/18(T)与康氏假单胞菌在物种水平上有明显区分。此外，根据16S rRNA基因序列，将核糖体印记图谱与新的禾本科植物分离株旁边聚类的假单胞菌物种进行比较，支持了它们在系统发育水平上的独立物种地位。基于表型特征，新分离株也可与其他具有精氨酸双水解酶和氧化酶反应阳性的荧光假单胞菌物种区分开来。这些表型和系统发育分析的结果表明，禾本科植物叶际分离株代表了一个新物种，为此提出了新种名 lurida假单胞菌（Pseudomonas lurida sp. nov.）。模式菌株为P 513/18(T)（=DSM 15835(T)=LMG 21995(T)）。
