Fan Jing, Guo Hui-Qin, Feng Su-Ling
School of Chemistry and Environmental Science, Henan Key Laboratory for Environmental, Pollution Control, Henan Normal University, Xinxiang, Henan 453007, P.R. China.
J Fluoresc. 2007 May;17(3):257-64. doi: 10.1007/s10895-007-0167-3. Epub 2007 Mar 29.
Hydroxyl radicals that is generated by Fenton reagent reacts with rhodamine B, which makes the fluorescence quenching of rhodamine B. However, there is an inhibitory effect of pentachlorophenol on the reaction. Based on this observation, an inhibitory fluorimetric method is reported for the determination of trace pentachlorophenol. The fluorescent inhibition of rhodamine B is measured by fix-time method. On the optimum conditions of experimentation, the detection limit for pentachlorophenol is 3.0 ng/ml and the linear range of the determination is 4.0-240 ng/ml. Combined with the samples treating with ion exchange resins and XDA-1 absorption resin, the method has been used for the determination of pentachlorophenol in synthetic samples and natural water samples with satisfactory results. We have also discussed the possible mechanism of the reaction.
由芬顿试剂产生的羟基自由基与罗丹明B发生反应，导致罗丹明B荧光猝灭。然而，五氯苯酚对该反应具有抑制作用。基于此观察结果，报道了一种用于测定痕量五氯苯酚的抑制荧光法。罗丹明B的荧光抑制通过固定时间法进行测定。在最佳实验条件下，五氯苯酚的检出限为3.0 ng/ml，测定线性范围为4.0 - 240 ng/ml。结合离子交换树脂和XDA - 1吸附树脂处理样品，该方法已用于合成样品和天然水样中五氯苯酚的测定，结果令人满意。我们还讨论了该反应可能的机理。
