Khan Munir H, Ali S, Fakhru'l-Razi A, Alam Z
Department of Chemical and Environmental Engineering, Universiti Putra Malaysia, Selangor D. E., Malaysia.
J Environ Sci Health B. 2007 May;42(4):381-6. doi: 10.1080/03601230701312647.
Cellulase production was carried out by solid state bioconversion (SSB) method using rice straw, a lignocellulosic material and agricultural waste, as the substrate of three Trichoderma spp. and Phanerochaete chrysosporium in lab-scale experiments. The results were compared to select the best fungi among them for the production of cellulase. Phanerochaete chrysosporium was found to be the best among these species of fungi, which produced the highest cellulase enzyme of 1.43 IU/mL of filter paper activity (FPase) and 2.40 IU/mL of carboxymethylcellulose activity (CMCase). The "glucosamine" and "reducing sugar" parameters were observed to evaluate the growth and substrate utilization in the experiments. In the case of Phanerochaete Chrysosporium, the highest glucosamine concentration was 1.60 g/L and a high concentration of the release of reducing sugar was measured as 2.58 g/L obtained on the 4th day of fermentation. The pH values were also recorded. The range of the pH was about 5.15 to 5.56 in the case of Phanerochaete Chrysosporium.
采用固态生物转化（SSB）方法，以木质纤维素材料稻草（一种农业废弃物）作为三种木霉属真菌和黄孢原毛平革菌的底物，在实验室规模的实验中进行纤维素酶的生产。对结果进行比较，以从中选出生产纤维素酶的最佳真菌。结果发现，在这些真菌中，黄孢原毛平革菌是最佳选择，其产生的纤维素酶活性最高，滤纸酶活性（FPase）为1.43 IU/mL，羧甲基纤维素酶活性（CMCase）为2.40 IU/mL。在实验中观察“氨基葡萄糖”和“还原糖”参数，以评估生长情况和底物利用情况。就黄孢原毛平革菌而言，最高氨基葡萄糖浓度为1.60 g/L，在发酵第4天测得还原糖释放的高浓度为2.58 g/L。还记录了pH值。黄孢原毛平革菌的pH范围约为5.15至5.56。
