Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011, USA.
J Agric Food Chem. 2012 Aug 8;60(31):7702-9. doi: 10.1021/jf301674u. Epub 2012 Jul 30.
Two agro-industrial coproducts, soybean cotyledon fiber and distiller's dried grains with solubles (DDGS), were used as substrates to evaluate the effect of coculturing three different fungi, Aspergillus oryzae, Trichoderma reesei, and Phanerochaete chrysosporium, on enzyme production by solid-state fermentation (SSF). When soybean fiber was used as the substrate, a maximum xylanase activity of 757.4 IU/g and a cellulase activity of 3.2 IU/g were achieved with the inoculation and incubation of T. reesei and P. chrysosporium for 36 h, followed by A. oryzae for an additional 108 h. This inoculation scheme also resulted in the highest xylanase activity of 399.2 IU/g compared to other fungi combinations in the SSF of DDGS. A large-scale SSF by this fungus combination produced fermented products that had xylanase and cellulase activities of 35.9-57.0 and 0.4-1.2 IU/g, respectively. These products also had 3.5-15.1% lower fiber and 1.3-4.2% higher protein contents, suggesting a potential feed quality improvement.
两种农业工业共生物，大豆子叶纤维和酒糟干燥可溶物（DDGS），被用作基质来评估共培养三种不同真菌（米曲霉、里氏木霉和黄孢原毛平革菌）对固态发酵（SSF）中酶生产的影响。当大豆纤维作为基质时，通过接种和培养里氏木霉和黄孢原毛平革菌 36 小时，然后再接种米曲霉 108 小时，可获得最高的木聚糖酶活性为 757.4 IU/g 和纤维素酶活性为 3.2 IU/g。与 SSF 中其他真菌组合相比，这种接种方案还导致 DDGS 的木聚糖酶活性最高，为 399.2 IU/g。通过这种真菌组合进行大规模 SSF 生产的发酵产物的木聚糖酶和纤维素酶活性分别为 35.9-57.0 和 0.4-1.2 IU/g。这些产物的纤维含量也分别降低了 3.5-15.1%，蛋白质含量提高了 1.3-4.2%，表明其具有改善饲料质量的潜力。
