Gabaĭ V L, Mosin A F
Biokhimiia. 1991 Sep;56(9):1652-60.
In Hank's balanced salt solution EL-4 ascites thymoma cells possessed endogenous respiration which was sufficient for the maintenance of their ATP level: pH decrease down to 6.0 had no effect either on endogenous respiration or the ATP level. Glucose had no influence on the respiration of EL-4 cells but inhibited that of Ehrlich ascites carcinoma (EAC) cells by 40% (Crabtree effect); respiration of the both cell lines was strongly (4-fold) inhibited after simultaneous addition of glucose, lactate and pH decrease. EL-4 cells had no endogenous glycolysis; EAC cells showed a low level of glycolysis only after pH decrease. Glucose addition led to activation of glycolysis (both inhibited 2-fold after a decrease of pH down to 6.0. The respiration inhibition at pH 7.3 and 6.0 caused no decrease of ATP depletion when glucose was present in the medium; this result may be due to suppression of ATP consumption. Incubation of EL-4 cells under respiration and glycolysis deficiency conditions resulted in a sharp ATP depletion; pH decrease delayed this depletion.
在汉克平衡盐溶液中，EL-4腹水胸腺瘤细胞具有内源性呼吸，足以维持其ATP水平：pH降至6.0对内源性呼吸或ATP水平均无影响。葡萄糖对EL-4细胞的呼吸没有影响，但可使艾氏腹水癌（EAC）细胞的呼吸抑制40%（克奈特效应）；同时添加葡萄糖、乳酸和降低pH后，两种细胞系的呼吸均受到强烈抑制（4倍）。EL-4细胞没有内源性糖酵解；EAC细胞仅在pH降低后显示出低水平的糖酵解。添加葡萄糖导致糖酵解激活（pH降至6.0后两者均被抑制2倍）。当培养基中存在葡萄糖时，pH 7.3和6.0时的呼吸抑制并未导致ATP消耗减少；这一结果可能是由于ATP消耗受到抑制。在呼吸和糖酵解缺乏的条件下培养EL-4细胞会导致ATP急剧消耗；pH降低会延迟这种消耗。
