磷脂酰肌醇-4-磷酸5'激酶（ppk-1）在秀丽隐杆线虫排卵中的作用。
Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans.
作者信息
Xu Xiaojian, Guo Haisu, Wycuff Diane L, Lee Myeongwoo
机构信息
Department of Biology, Baylor University, One Bear Place 97388, Waco, TX 76798, USA.
出版信息
Exp Cell Res. 2007 Jul 1;313(11):2465-75. doi: 10.1016/j.yexcr.2007.03.017. Epub 2007 Mar 24.
Abstract
During Caenorhabditis elegans ovulation, the somatic gonad integrates signals from germ cells and propels a mature oocyte into the spermatheca for fertilization. Previous work suggests that phosphoinositide signaling plays important roles in C. elegans fertility. To fully understand inositol-1,4,5-trisphosphate (IP(3)) signaling in ovulation, we have examined the function of phosphatidylinositol-4-phosphate 5' kinase (PIP5K) in C. elegans. Our results show that the C. elegans PIP5K homolog, ppk-1, is essential for ovulation in C. elegans; ppk-1 is mainly expressed in somatic gonad, and depletion of ppk-1 expression causes defective ovulation, reduced gonad sheath contractility, and sterility. Increased IP(3) signaling compensates for ppk-1 (RNAi)-induced sterility, suggesting that ppk-1 is linked to IP(3) signaling. These results demonstrate that ppk-1 plays an essential role in IP(3) signaling and cytoskeleton organization in somatic gonad.
摘要
在秀丽隐杆线虫排卵过程中，体细胞性腺整合来自生殖细胞的信号，并将成熟的卵母细胞推进受精囊进行受精。先前的研究表明，磷酸肌醇信号传导在秀丽隐杆线虫的生育能力中起重要作用。为了全面了解排卵过程中肌醇-1,4,5-三磷酸（IP(3)）信号传导，我们研究了秀丽隐杆线虫中磷脂酰肌醇-4-磷酸5'激酶（PIP5K）的功能。我们的结果表明，秀丽隐杆线虫PIP5K同源物ppk-1对秀丽隐杆线虫的排卵至关重要；ppk-1主要在体细胞性腺中表达，ppk-1表达的缺失会导致排卵缺陷、性腺鞘收缩力降低和不育。IP(3)信号传导的增加可补偿ppk-1（RNA干扰）诱导的不育，这表明ppk-1与IP(3)信号传导有关。这些结果表明，ppk-1在体细胞性腺的IP(3)信号传导和细胞骨架组织中起重要作用。
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