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克氏锥虫：一种在寄生虫生命周期中差异表达的RNA结合蛋白的分子特征

Trypanosoma cruzi: molecular characterization of an RNA binding protein differentially expressed in the parasite life cycle.

作者信息

Pérez-Díaz Leticia, Duhagon María Ana, Smircich Pablo, Sotelo-Silveira José, Robello Carlos, Krieger Marco Aurelio, Goldenberg Samuel, Williams Noreen, Dallagiovanna Bruno, Garat Beatriz

机构信息

Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay.

出版信息

Exp Parasitol. 2007 Sep;117(1):99-105. doi: 10.1016/j.exppara.2007.03.010. Epub 2007 Mar 27.

DOI:10.1016/j.exppara.2007.03.010
PMID:17475252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2020836/
Abstract

Molecular studies have shown several peculiarities in the regulatory mechanisms of gene expression in trypanosomatids. Protein coding genes are organized in long polycistronic units that seem to be constitutively transcribed. Therefore, post-transcriptional regulation of gene expression is considered to be the main point for control of transcript abundance and functionality. Here we describe the characterization of a 17 kDa RNA-binding protein from Trypanosoma cruzi (TcRBP19) containing an RNA recognition motive (RRM). This protein is coded by a single copy gene located in a high molecular weight chromosome of T. cruzi. Orthologous genes are present in the TriTryp genomes. TcRBP19 shows target selectivity since among the different homoribopolymers it preferentially binds polyC. TcRBP19 is a low expression protein only barely detected at the amastigote stage localizing in a diffuse pattern in the cytoplasm.

摘要

分子研究表明，锥虫基因表达调控机制存在一些独特之处。蛋白质编码基因以长的多顺反子单元形式组织，似乎是组成型转录的。因此，基因表达的转录后调控被认为是控制转录本丰度和功能的关键环节。在此，我们描述了来自克氏锥虫的一种17 kDa RNA结合蛋白（TcRBP19）的特性，该蛋白含有一个RNA识别基序（RRM）。该蛋白由位于克氏锥虫一条高分子量染色体上的单拷贝基因编码。在三锥虫基因组中存在直系同源基因。TcRBP19表现出靶标选择性，因为在不同的同聚核糖聚合物中，它优先结合聚C。TcRBP19是一种低表达蛋白，仅在无鞭毛体阶段勉强可检测到，定位于细胞质中呈弥散状。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/127e8ed68aae/nihms29099f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/4594b8580ebb/nihms29099f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/5871cf8499e2/nihms29099f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/bf9ce60c7c8b/nihms29099f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/24761d90db28/nihms29099f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/127e8ed68aae/nihms29099f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/4594b8580ebb/nihms29099f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/5871cf8499e2/nihms29099f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/bf9ce60c7c8b/nihms29099f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/24761d90db28/nihms29099f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d228/2020836/127e8ed68aae/nihms29099f5.jpg

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本文引用的文献

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RNA-binding domain proteins in Kinetoplastids: a comparative analysis.动质体中的RNA结合结构域蛋白：比较分析
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The genome of the kinetoplastid parasite, Leishmania major.动质体寄生虫硕大利什曼原虫的基因组。
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The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease.克氏锥虫（恰加斯病的病原体）的基因组序列。
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Protein interactions within the TcZFP zinc finger family members of Trypanosoma cruzi: implications for their functions.克氏锥虫TcZFP锌指家族成员内的蛋白质相互作用：对其功能的影响。
Biochem Biophys Res Commun. 2005 Aug 5;333(3):1017-25. doi: 10.1016/j.bbrc.2005.06.007.
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Trypanosoma cruzi: molecular characterization of TcPUF6, a Pumilio protein.克氏锥虫：一种Pumilio蛋白TcPUF6的分子特征
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