Amiya Noriko, Amano Masafumi, Yamanome Takeshi, Yamamori Kunio, Takahashi Akiyoshi
School of Fisheries Sciences, Kitasato University, Ofunato, Iwate 022-0101, Japan.
Gen Comp Endocrinol. 2008 Jan 1;155(1):88-93. doi: 10.1016/j.ygcen.2007.03.007. Epub 2007 Mar 24.
Effects of background color on gonadotropin-releasing hormone (GnRH) and melanin-concentrating hormone (MCH) levels in the brain of the barfin flounder Verasper moseri were monitored to investigate the interaction of GnRH and MCH in the brain. Fish were reared in white or black tanks from one month after hatching for about 7 months. MCH levels in the brain and pituitary were higher in the white tank fish. In contrast, chicken GnRH-II (cGnRH-II) levels in the brain were higher in the black tank fish. No significant differences between background colors were observed in the brain concerning salmon GnRH and seabream GnRH levels. Furthermore, six-month-old fish that had been reared in white tank were transferred to another white or black tank. Brain cGnRH-II levels were higher in black tank fish than those in white tank at 2 and 7 days after the transfer. Double-staining immunohistochemistry showed that some cGnRH-II-immunoreactive (ir) fibers were in close contact with MCH-ir cell bodies in the hypothalamus. These results indicate that background color affects not only MCH levels but also cGnRH-II levels in the brain and suggest that cGnRH-II may play a role in the regulation of MCH neural function, food intake, in the brain of the barfin flounder.
为了研究牙鲆（Verasper moseri）脑中促性腺激素释放激素（GnRH）和促黑素细胞激素（MCH）之间的相互作用，监测了背景颜色对牙鲆脑中GnRH和MCH水平的影响。从孵化后一个月起，将牙鲆饲养在白色或黑色水箱中约7个月。白色水箱中的牙鲆脑和垂体中的MCH水平较高。相反，黑色水箱中的牙鲆脑中鸡GnRH-II（cGnRH-II）水平较高。关于脑中鲑鱼GnRH和鲷鱼GnRH水平，未观察到背景颜色之间的显著差异。此外，将饲养在白色水箱中的6月龄牙鲆转移到另一个白色或黑色水箱中。转移后2天和7天时，黑色水箱中的牙鲆脑cGnRH-II水平高于白色水箱中的牙鲆。双重免疫组织化学染色显示，下丘脑中有一些cGnRH-II免疫反应性（ir）纤维与MCH-ir细胞体紧密接触。这些结果表明，背景颜色不仅影响脑中的MCH水平，还影响cGnRH-II水平，并表明cGnRH-II可能在牙鲆脑的MCH神经功能调节和食物摄入中发挥作用。
