Karppi Jouni, Akerman Satu, Akerman Kari, Sundell Annika, Nyyssönen Kristiina, Penttilä Ilkka
Laboratory Centre, Kuopio University Hospital, Kuopio, Finland.
Eur J Pharm Biopharm. 2007 Sep;67(2):562-8. doi: 10.1016/j.ejpb.2007.03.014. Epub 2007 Mar 25.
Isolation of acidic and basic model drugs by using pH sensitive poly(acrylic acid) grafted poly(vinylidene fluoride) (PAA-PVDF) cation-exchange membrane from biological fluids was reported. Effects of drug charge and lipophilicity on adsorption were also investigated. In the present study, basic model drugs adsorbed to a considerably greater extent onto the membrane than acidic drugs. Albumin was not adsorbed onto the membrane. Results of our study exposed, that electrostatic interactions between positively charged basic drug and negatively charged PVDF-PAA membrane were the most important factor affecting drug adsorption onto the membrane. Adsorption of acidic and basic drugs onto the PVDF-PAA membrane was not related to drug lipophilicity. The results of present study demonstrated that basic drugs adsorbed extensively onto the membrane, but albumin did not, proposing that PAA-PVDF membrane may be suitable for isolating basic drugs from proteinaceous biological fluids (i.e. serum) for subsequent monitoring and evaluation.
据报道，使用pH敏感的聚丙烯酸接枝聚偏氟乙烯（PAA-PVDF）阳离子交换膜从生物流体中分离酸性和碱性模型药物。还研究了药物电荷和亲脂性对吸附的影响。在本研究中，碱性模型药物在膜上的吸附程度比酸性药物大得多。白蛋白未吸附在膜上。我们的研究结果表明，带正电荷的碱性药物与带负电荷的PVDF-PAA膜之间的静电相互作用是影响药物在膜上吸附的最重要因素。酸性和碱性药物在PVDF-PAA膜上的吸附与药物亲脂性无关。本研究结果表明，碱性药物在膜上广泛吸附，但白蛋白不吸附，这表明PAA-PVDF膜可能适用于从含蛋白质的生物流体（即血清）中分离碱性药物，以便后续监测和评估。
