Amirkhanian O M, Amirkhanian L T, Karagezian K G
Biokhimiia. 1975 Sep-Oct;40(5):990-2.
A simple modification of the method of Rossiter and Palmer for the quantitative determination of phosphoinositides (PI) in animal tissues (brain and liver) is worked out: Phospholipids are mineralyzed (together with a paper) in 57% perchloric acid, avoiding the time consuming procedure of the tenfold elution of inositids spots from chromatogramms and the subsequent evaporation of the substantial volume of the eluate obtained. The time of the analysis is twice as short as that of the original method, the data obtained being reliable. The quantity of mono-, di- and triphosphoinositides in rat brain is as follows: 85.08, 11.33 and 32.85 mkg P/g wet tissue, but in liver it is 94.5, 1.88 and 2.5 mkg P/g wet tissue respectively.
已制定出一种对罗斯特和帕尔默方法的简单改进，用于定量测定动物组织（脑和肝）中的磷酸肌醇（PI）：磷脂（与一张纸一起）在57%的高氯酸中进行矿化，避免了从色谱图中对肌醇斑点进行十次洗脱以及随后蒸发大量洗脱液这一耗时的过程。分析时间比原方法缩短了一半，所得数据可靠。大鼠脑中单磷酸肌醇、二磷酸肌醇和三磷酸肌醇的含量如下：85.08、11.33和32.85微克磷/克湿组织，但在肝脏中分别为94.5、1.88和2.5微克磷/克湿组织。
