Araki Tetsuro, Kusakabe Morioh, Nishida Eisuke
Department of Cell and Developmental Biology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
Gene Expr Patterns. 2007 Jun;7(6):666-71. doi: 10.1016/j.modgep.2007.03.004. Epub 2007 Mar 30.
Estrogen induced gene 121 (EIG121) and EIG121-like (EIG121L) are evolutionarily conserved genes. But, their function is still unknown. Here, we report the expression pattern of Xenopus EIG121-like (xEIG121L) during early development. Its expression was first detected at stage 9 after mid-blastula transition, attained its maximal level at the gastrula stage, and remained constant until the tadpole stage. Whole-mount in situ hybridization revealed that xEIG121L was expressed strongly in the ventral ectoderm at the gastrula stage, and in the anterior ectoderm surrounding the neural plate at the neurula stage. xEIG121L expression was especially high in the presumptive hatching gland and cement gland regions in the neurula. At the tailbud stage, xEIG121L expression was limited to the hatching gland; an inverted Y type staining, characteristic of the hatching gland, was observed. However, at the tadpole stage, xEIG121L was expressed broadly in the head, heart and fin.
雌激素诱导基因121（EIG121）和类EIG121（EIG121L）是进化保守基因。但是，它们的功能仍然未知。在此，我们报道了非洲爪蟾类EIG121（xEIG121L）在早期发育过程中的表达模式。其表达在囊胚中期转换后的第9期首次被检测到，在原肠胚期达到最高水平，并一直保持到蝌蚪期。整体原位杂交显示，xEIG121L在原肠胚期的腹侧外胚层强烈表达，在神经胚期围绕神经板的前侧外胚层表达。xEIG121L在神经胚期的预定孵化腺和黏腺区域表达特别高。在尾芽期，xEIG121L的表达局限于孵化腺；观察到一种孵化腺特有的倒Y型染色。然而，在蝌蚪期，xEIG121L在头部、心脏和鳍中广泛表达。
