Kireev R A, Tresguerres A F, Vara E, Ariznavarreta C, Tresguerres J A F
Department of Biochemistry and Biophysics, Saratov State University, Saratov, Russia.
Biogerontology. 2007 Oct;8(5):469-82. doi: 10.1007/s10522-007-9089-3. Epub 2007 May 3.
The aging theory postulates that this process may be due to the accumulation of oxidative damage in cells and molecules. The present study has investigated the effect of castration in old female rats on various parameters related to the antioxidant properties of several cellular fractions obtained from the liver, and the influence of several chronic treatments on it, both in intact and castrated animals. Sixty-one 22-month-old Wistar female rats, were used. About 21 intact animals were divided into three groups and treated for 10 weeks with GH, melatonin or saline, and 40 ovariectomized (at 12 months of age) animals were divided into five groups and treated for the same time with GH, melatonin, estrogens (Eos), phytoestrogens (Phyt) or saline. All animals were sacrificed at 24 months of age by decapitation. The activity of glutathione peroxidase (GPx) in cytosolic fraction, glutathione-S-transferase (GST) in cytosol and microsomal fractions, and the levels of nitric oxide (NO) and cytochrome C in mitochondrial and cytosol fractions of liver were determined. A decrease in GST activity was detected in cytosol and in the microsomal fraction in ovariectomized animals as compared to intact rats. The activity of GPx was also decreased in ovariectomized as compared with the intact group. NO level was increased and cytochrome C decreased in the mitochondrial fraction of the liver in ovariectomized females as compared with the intact group, respectively. No significant changes after melatonin or GH treatments were found in GPx, GST activity and NO level in mitochondrial fraction in the intact group. Administration of GH, melatonin, Eos and Phyt in the ovariectomized groups significantly increased the GPx, and GST activity in the cytosol and microsomal fraction and decreased the level of NO in the mitochondrial fraction as compared with the untreated rats. A significant increase in the level of cytochrome C in the mitochondrial fraction and a decrease in the cytosol fraction were also found with all treatments. The administration of GH, melatonin, Eos and Phyt to castrated females seem to reduce oxidative changes in the liver from old ovariectomized rats.
衰老理论假定，这一过程可能是由于细胞和分子中氧化损伤的积累所致。本研究调查了老年雌性大鼠去势对从肝脏获得的几种细胞组分抗氧化特性相关的各种参数的影响，以及在完整和去势动物中几种慢性治疗对其的影响。使用了61只22月龄的Wistar雌性大鼠。约21只完整动物被分为三组，分别用生长激素（GH）、褪黑素或生理盐水处理10周，40只（12月龄时）卵巢切除的动物被分为五组，同时用GH、褪黑素、雌激素（Eos）、植物雌激素（Phyt）或生理盐水处理。所有动物在24月龄时断头处死。测定了肝脏胞质组分中谷胱甘肽过氧化物酶（GPx）的活性、胞质和微粒体组分中谷胱甘肽-S-转移酶（GST）的活性，以及线粒体和胞质组分中一氧化氮（NO）和细胞色素C的水平。与完整大鼠相比，卵巢切除动物的胞质和微粒体组分中GST活性降低。与完整组相比，卵巢切除动物中GPx的活性也降低。与完整组相比，卵巢切除雌性大鼠肝脏线粒体组分中NO水平升高，细胞色素C降低。完整组中，褪黑素或GH处理后，GPx、GST活性和线粒体组分中NO水平无显著变化。与未处理大鼠相比，卵巢切除组中给予GH、褪黑素、Eos和Phyt显著增加了胞质和微粒体组分中GPx和GST的活性，并降低了线粒体组分中NO的水平。所有处理还使线粒体组分中细胞色素C水平显著升高，胞质组分中细胞色素C水平降低。对去势雌性大鼠给予GH、褪黑素、Eos和Phyt似乎可减少老年卵巢切除大鼠肝脏中的氧化变化。
