Zhang Guodong, Ma Li, Doyle Michael P
Center for Food Safety, 1109 Experiment Street, University of Georgia, Griffin, Georgia 30223, USA.
J Food Prot. 2007 Apr;70(4):874-8. doi: 10.4315/0362-028x-70.4.874.
The objective of this study was to develop a defined competitive exclusion bacteria (CE) culture that will prevent or substantially reduce Salmonella colonization of poultry. The efficacy of 56 potential CE isolates in preventing or reducing Salmonella colonization in chickens was determined. These potential CE were perorally administered to day-of-hatch chicks at 10(6) to 10(8) CFU per chick, and salmonellae were subsequently administered by gavage 2 days later at 5.5 x 10(3) to 5.0 x 10(4) CFU per chick. Feeding chickens an overnight CE culture of Lactobacillus salivarius strains Salm-9, List40-1,8, or List40-41 reduced Salmonella carriage in cecal contents by 2.10, 2.52, and 2.20 log CFU/g (average of three trials), respectively. The percentages of Salmonella-positive chickens after receiving these treatments were 35, 31, and 35% respectively, compared with 84% for the untreated control. A mixture of these three isolates had a similar effect when compared with the results of the individual isolates. A mixture of Streptococcus cristatus List40-13 and L. salivarius List40-41 reduced Salmonella carriage from 90 to 65% and 88 to 31% in two feeding trials, and by 2.2 and 4 log CFU/g of cecal contents of chickens. In conclusion, CE isolates L. salivarius Salm-9, List40-18, and List40-41 and S. cristatus List40-13 either individually or in combination were effective in significantly preventing Salmonella colonization of chickens.
本研究的目的是开发一种特定的竞争性排斥细菌（CE）培养物，以预防或大幅减少家禽沙门氏菌的定植。测定了56种潜在CE分离株在预防或减少鸡沙门氏菌定植方面的功效。将这些潜在的CE以每只雏鸡10(6)至10(8)CFU的剂量经口给予刚出壳的雏鸡，随后在2天后通过灌胃给予每只雏鸡5.5×10(3)至5.0×10(4)CFU的沙门氏菌。给鸡喂食唾液乳杆菌菌株Salm-9、List40-1、8或List40-41的过夜CE培养物，分别使盲肠内容物中的沙门氏菌携带量降低了2.10、2.52和2.20 log CFU/g（三次试验的平均值）。接受这些处理后沙门氏菌呈阳性的鸡的百分比分别为35%、31%和35%，而未处理的对照组为84%。与单个分离株的结果相比，这三种分离株的混合物具有类似的效果。在两项饲养试验中，冠突链球菌List40-13和唾液乳杆菌List40-41的混合物使沙门氏菌携带率从90%降至65%，从88%降至31%，并使鸡盲肠内容物中的沙门氏菌携带量分别降低了2.2和4 log CFU/g。总之，CE分离株唾液乳杆菌Salm-9、List40-18和List40-41以及冠突链球菌List40-13单独或联合使用均能有效显著预防鸡的沙门氏菌定植。
