Koul Ashwani, Binepal Gursonika, Gangar Subhash Chander
Department of Biophysics, Basic Medical Sciences Block, Panjab University, Chandigarh 160 014, India.
Indian J Exp Biol. 2007 Apr;45(4):359-66.
Considering the hepatoprotective properties of Azadirachta indica, the present study was designed to evaluate its preventive effects against diethylnitrosamine (NDEA) induced hepatotoxicity in male Balb/c mice. Exposure of NDEA caused a significant increase in micronucleated cell score, lipid peroxidation levels (LPO) and activity of lactate dehydrogenase (LDH). A significant decrease in reduced glutathione (GSH) contents and activity of glutathione-S-transferase (GST) was also observed upon NDEA treatment, whereas their activities of cytochrome P450 and cytochrome b5 showed non-significant alterations. Aqueous A. indica leaf extract (AAILE) pretreatment showed protective effects against NDEA induced toxicity by decreasing the frequency of micronucleated cell, levels of LPO and LDH activity. Also, a decreased activity of GST, cytochrome P450 and an increased activity of cytochrome b5, GSH contents was observed when AAILE pretreated mice were injected with NDEA. Only AAILE treatment caused a noticeable decrease in the frequency of micronuclei, activity of cytochrome P450 and cytochrome b5, but a significant increase in the activity of GST and GSH contents, whereas, non significant alterations were observed in the activity of LDH and levels of LPO. Significance of these observations with respect to hepatoprotective efficacy of A. indica has been discussed in the present manuscript.
鉴于印楝的肝脏保护特性，本研究旨在评估其对雄性Balb/c小鼠二乙基亚硝胺（NDEA）诱导的肝毒性的预防作用。NDEA暴露导致微核细胞 score、脂质过氧化水平（LPO）和乳酸脱氢酶（LDH）活性显著增加。NDEA处理后还观察到还原型谷胱甘肽（GSH）含量和谷胱甘肽 - S - 转移酶（GST）活性显著降低，而细胞色素P450和细胞色素b5的活性无显著变化。印楝叶水提取物（AAILE）预处理通过降低微核细胞频率、LPO水平和LDH活性，对NDEA诱导的毒性显示出保护作用。此外，当用NDEA注射AAILE预处理的小鼠时，观察到GST、细胞色素P450活性降低，细胞色素b5、GSH含量活性增加。仅AAILE处理导致微核频率、细胞色素P450和细胞色素b5活性显著降低，但GST活性和GSH含量显著增加，而LDH活性和LPO水平无显著变化。本手稿讨论了这些观察结果对印楝肝脏保护功效的意义。
