Lavhale Manish S, Mishra S H
Pharmacy Department, Faculty of Technology and Engineering, The M.S. University of Baroda, Vadodara 390 002, India.
Indian J Exp Biol. 2007 Apr;45(4):376-84.
In the present study, ethyl acetate, butanol and aqueous fractions derived from total methanol extract of Butea monosperma flowers were evaluated for radical scavenging activities using different in vitro models like reducing power assay, scavenging of 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical, nitric oxide radical, superoxide anion radical, hydroxyl radical and inhibition of erythrocyte hemolysis using 2, 2' azo-bis (amidinopropane) dihydrochloride (AAPH). Methanol extract along with its ethyl acetate and butanol fractions showed potent free radical scavenging activity, whereas aqueous fraction was found to be devoid of any radical scavenging properties. The observed activity could be due to the higher phenolic content in the extracts (16.1, 25.29, and 17.74% w/w in methanol extract, ethyl acetate and butanol fractions respectively). HPTLC fingerprint profile of the ethyl acetate and butanol fractions were developed which would serve as reference standard for quality control of the extracts.
在本研究中，使用不同的体外模型，如还原能力测定、2,2-二苯基-1-苦基肼（DPPH）自由基清除、一氧化氮自由基清除、超氧阴离子自由基清除、羟自由基清除以及使用2,2'-偶氮双（脒丙烷）二盐酸盐（AAPH）抑制红细胞溶血，对紫铆花总甲醇提取物的乙酸乙酯、丁醇和水相部分进行了自由基清除活性评估。甲醇提取物及其乙酸乙酯和丁醇部分表现出强大的自由基清除活性，而水相部分则没有任何自由基清除特性。观察到的活性可能归因于提取物中较高的酚类含量（甲醇提取物、乙酸乙酯和丁醇部分中的酚类含量分别为16.1%、25.29%和17.74% w/w）。开发了乙酸乙酯和丁醇部分的高效薄层色谱（HPTLC）指纹图谱，这将作为提取物质量控制的参考标准。
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