Bell S E, Zamoyska R
Department of Biology, University College, London, Great Britain.
Eur J Immunol. 1991 Dec;21(12):2931-6. doi: 10.1002/eji.1830211206.
We have isolated thymic stem cells from murine fetal liver using a combination of approaches including discontinuous albumin density gradient separation and anti-heat-stable antigen (HSA) antibody plus complement-mediated lysis. A population of early precursors has been identified which comprises approximately 1% of fetal liver, and which has the capacity to reconstitute alymphoid lobes and give rise to the four thymic subpopulations defined by expression of the T cell differentiation antigens CD4 and CD8. These early precursors express CD44 (Pgp-1), however, they are negative for expression of Thy-1, CD4, CD8, CD3, membrane immunoglobulin and HSA cell surface molecules.
我们采用包括不连续白蛋白密度梯度分离、抗热稳定抗原（HSA）抗体加补体介导的细胞溶解等多种方法，从小鼠胎肝中分离出胸腺干细胞。已鉴定出一群早期前体细胞，其约占胎肝的1%，能够重建无淋巴细胞的叶，并产生由T细胞分化抗原CD4和CD8的表达所定义的四个胸腺亚群。这些早期前体细胞表达CD44（Pgp-1），然而，它们不表达Thy-1、CD4、CD8、CD3、膜免疫球蛋白和HSA细胞表面分子。
