Bengtén E, Leanderson T, Pilström L
Department of Immunology, BMC, Uppsala University, Sweden.
Eur J Immunol. 1991 Dec;21(12):3027-33. doi: 10.1002/eji.1830211219.
Rabbit antibodies to Atlantic cod (Gadus morhua L.) immunoglobulin were affinity purified and used to screen cDNA libraries from spleen and head kidney mRNA. cDNA clones for both the secretory and membrane-bound heavy (H) chain were isolated, the nucleotide and deduced amino acid sequences of which are reported here. Comparisons of the cod secretory H chain amino acid sequence show 24%, 27%, 30% identity to the mu chain of Mus, Xenopus and Ictalurus, respectively. The highest degree of identity was observed in the CH4 domain. The cDNA encoding the transmembrane form shows a novel splicing pattern where the TM1 exon is spliced directly onto the CH3 domain and not to the CH4 domain as in other animal groups. Southern blot analyses with VH and C probes on genomic DNA from cod erythrocytes indicate that there is a unique C gene but several V genes in the cod immunoglobulin H chain locus.
针对大西洋鳕鱼（Gadus morhua L.）免疫球蛋白的兔抗体经亲和纯化后，用于筛选来自脾脏和头肾mRNA的cDNA文库。分离出了分泌型和膜结合型重链（H链）的cDNA克隆，本文报道了其核苷酸和推导的氨基酸序列。鳕鱼分泌型H链氨基酸序列与小家鼠、非洲爪蟾和斑点叉尾鮰的μ链的同一性分别为24%、27%、30%。在CH4结构域观察到最高程度的同一性。编码跨膜形式的cDNA显示出一种新的剪接模式，其中TM1外显子直接剪接到CH3结构域，而不像其他动物群体那样剪接到CH4结构域。用VH和C探针对鳕鱼红细胞基因组DNA进行Southern印迹分析表明，在鳕鱼免疫球蛋白H链基因座中有一个独特的C基因和几个V基因。
