Fujiyama Kazuhito, Furukawa Aiko, Katsura Atsuya, Misaki Ryo, Omasa Takeshi, Seki Tatsuji
The International Center for Biotechnology, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2007 Jun 22;358(1):85-91. doi: 10.1016/j.bbrc.2007.04.054. Epub 2007 Apr 19.
Previously, we developed a transgenic tobacco BY2 cell line (GT6) in which glycosylation was modified by expressing human beta(1,4)-galactosyltransferase (betaGalT). In this study, we produced a mouse monoclonal antibody in GT6 cells, and determined the sugar chain structures of plant-produced antibodies. Galactose-extended N-linked glycans comprised 16.7%, and high-mannose-type and complex-type glycans comprised 38.5% and 35.0% of the total number of glycans, respectively. N-linked glycans with the plant-specific sugars beta(1,2)-xylose and alpha(1,3)-fucose comprised 9.8%. The introduction of human betaGalT into suspension cultured tobacco cells resulted in the production of recombinant proteins with galactose-extended glycans and decreased contents of beta(1,2)-xylose and alpha(1,3)-fucose.
此前，我们构建了一种转基因烟草BY2细胞系（GT6），通过表达人β（1,4）-半乳糖基转移酶（βGalT）来修饰糖基化。在本研究中，我们在GT6细胞中制备了一种小鼠单克隆抗体，并确定了植物产生抗体的糖链结构。半乳糖延伸型N-聚糖占聚糖总数的16.7%，高甘露糖型和复合型聚糖分别占聚糖总数的38.5%和35.0%。含有植物特异性糖类β（1,2）-木糖和α（1,3）-岩藻糖的N-聚糖占9.8%。将人βGalT导入悬浮培养的烟草细胞中，导致产生具有半乳糖延伸型聚糖且β（1,2）-木糖和α（1,3）-岩藻糖含量降低的重组蛋白。
