Ganesh Kumar A, Swarnalatha S, Sairam B, Sekaran G
Department of Environmental Technology, Central Leather Research Institute, Adyar, Chennai, India.
Bioresour Technol. 2008 Apr;99(6):1939-44. doi: 10.1016/j.biortech.2007.03.025. Epub 2007 May 3.
Animal fleshing (ANFL), the major proteinaceous solid waste discharged from leather manufacturing industries was used as the substrate for the production of alkaline protease by Pseudomonas aeruginosa. The strain isolated from the tannery wastewater was selected for its ability to produce protease of activity in the range 1160-1175 U ml(-1). The selective removal of non-fibrillar proteins such as albumin and globulin from ANFL by the protease enzyme during the progress of hydrolysis was confirmed using scanning electron microscopy (SEM). The breakdown of ANFL was also confirmed from the amino acid release into the fermentation medium by P. aeruginosa using high performance liquid chromatography (HPLC).
动物皮屑（ANFL）是皮革制造行业排放的主要蛋白质类固体废物，被用作铜绿假单胞菌生产碱性蛋白酶的底物。从制革废水中分离出的菌株因其产生活性在1160 - 1175 U ml(-1)范围内的蛋白酶的能力而被选中。在水解过程中，利用扫描电子显微镜（SEM）证实了蛋白酶对ANFL中非纤维状蛋白质如白蛋白和球蛋白的选择性去除。通过高效液相色谱（HPLC）也证实了铜绿假单胞菌将氨基酸释放到发酵培养基中导致ANFL的分解。
