在有症状的三角纤维软骨中央撕裂中检测凋亡软骨细胞。

Detection of apoptotic cartilage cells in symptomatic central tears of the triangular fibrocartilage.

作者信息

Unglaub Frank, Fellenberg Jorg, Germann Guenter, Bickert Berthold, Sauerbier Michael, Richter Wiltrud

机构信息

Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center Ludwigshafen, Plastic and Hand Surgery of the University of Heidelberg, Ludwigshafen, Germany.

出版信息

J Hand Surg Am. 2007 May-Jun;32(5):618-22. doi: 10.1016/j.jhsa.2007.02.023.

DOI:10.1016/j.jhsa.2007.02.023

PMID:17481998

Abstract

PURPOSE

Central traumatic injuries of the triangular fibrocartilage are frequent sources of ulnar wrist pain. It is unknown whether the fibrocartilage cells survive this trauma in the central part of the triangular fibrocartilage. The goal of this study was to determine the viability of cells in traumatic triangular fibrocartilage complex (TFCC) Palmer 1A lesions to estimate the role of apoptosis for the fate of fibrocartilage cells.

METHODS

Twenty-two patients with a symptomatic central traumatic tear in the triangular fibrocartilage were included in this study. The cartilage was debrided arthroscopically, and histologic sections were used for the analysis of cell viability and apoptosis. Cell viability was quantified by terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, and apoptotic cells were visualized by poly(ADP-ribose) polymerase (PARP) p85 immunohistochemistry. The number of apoptotic cells was correlated with the total number of cells.

RESULTS

In contrast with the control patients with only minimal amounts of TUNEL-positive cells, cell viability was markedly reduced in all analyzed patients with TFCC Palmer 1A lesion. Likewise, the number of PARP p85-positive cells was significantly increased in all patients. This indicates that the observed cell death is attributed to apoptosis. Concerning the distribution of apoptotic cells, the inner zone of the biospy showed much higher numbers of apoptotic cells than the outer zone in both PARP and TUNEL staining. In contrast to the analyzed patients, no apoptosis could be detected in the controls.

CONCLUSIONS

High amounts of apoptotic cartilage cells could be detected within the inner part of the triangular fibrocartilage suggesting that fibrocartilage cells died in response to trauma and were not quickly replaced by new viable cells. PARP p85-positive cells indicate that the apoptotic cascade plays a crucial role in the TFCC Palmer 1A disorder.

摘要

目的

三角纤维软骨中央创伤性损伤是尺侧腕痛的常见原因。尚不清楚三角纤维软骨中央部分的纤维软骨细胞在这种创伤后是否存活。本研究的目的是确定创伤性三角纤维软骨复合体（TFCC）Palmer 1A损伤中细胞的活力，以评估细胞凋亡在纤维软骨细胞命运中的作用。

方法

本研究纳入了22例有症状的三角纤维软骨中央创伤性撕裂患者。通过关节镜清理软骨，并使用组织学切片分析细胞活力和凋亡情况。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法对细胞活力进行定量，通过聚（ADP-核糖）聚合酶（PARP）p85免疫组织化学观察凋亡细胞。将凋亡细胞数量与细胞总数进行关联分析。

结果

与仅存在少量TUNEL阳性细胞的对照患者相比，所有分析的TFCC Palmer 1A损伤患者的细胞活力均显著降低。同样，所有患者中PARP p85阳性细胞数量均显著增加。这表明观察到的细胞死亡归因于细胞凋亡。关于凋亡细胞的分布，在PARP和TUNEL染色中，活检标本的内部区域显示的凋亡细胞数量均远高于外部区域。与分析的患者不同，对照组未检测到细胞凋亡。