Victory Rahi, Saed Ghassan M, Diamond Michael P
Department of Obstetrics and Gynecology, C.S. Mott Center, Wayne State University, Detroit, Michigan 48201, USA.
Fertil Steril. 2007 Dec;88(6):1657-62. doi: 10.1016/j.fertnstert.2007.01.123. Epub 2007 May 4.
To determine whether docosahexaenoic acid (DHA) reduces adhesion marker mRNA levels in normal peritoneal and adhesion fibroblasts.
Prospective experimental study.
University Medical Center.
PATIENT(S): Three patients undergoing laparotomy with excision of adhesions and normal peritoneum.
INTERVENTION(S): DHA treatment (100 muM) of cell cultures for 24 hours.
MAIN OUTCOME MEASURE(S): Real-time reverse transcriptase polymerase chain reaction (RT-PCR) quantification of relative changes (mRNA copies/mug mRNA) in mRNA levels of type I collagen, vascular endothelial growth factor (VEGF), and transforming growth factor-beta1 (TGF-beta1).
RESULT(S): The DHA treatment significantly reduced type I collagen and VEGF, but not TGF-beta1 mRNA levels in normal peritoneal fibroblasts compared to normal controls. The DHA treatment of adhesion fibroblasts reduced type I collagen mRNAs to those of normal peritoneal fibroblasts, decreasing mRNAs by 35% compared to untreated adhesion fibroblasts. The VEGF mRNA levels were 50% lower in DHA-treated adhesion fibroblasts versus untreated adhesion fibroblasts. Docasahexaenoic acid reduced TGF- beta1 mRNA to normal levels in treated adhesion fibroblasts compared to untreated normal peritoneal fibroblasts.
CONCLUSION(S): Docasahexaenoic acid substantially reduces levels of adhesion-related markers in normal peritoneal and adhesion fibroblasts. This study provides the molecular basis for an easily administered and potentially, highly efficacious, antiadhesion adjuvant.
确定二十二碳六烯酸（DHA）是否能降低正常腹膜和成纤维细胞中粘连标志物的mRNA水平。
前瞻性实验研究。
大学医学中心。
3例接受粘连切除及正常腹膜切除的剖腹手术患者。
对细胞培养物进行24小时的DHA治疗（100μM）。
通过实时逆转录聚合酶链反应（RT-PCR）定量检测I型胶原蛋白、血管内皮生长因子（VEGF）和转化生长因子-β1（TGF-β1）mRNA水平的相对变化（mRNA拷贝数/μg mRNA）。
与正常对照组相比，DHA治疗显著降低了正常腹膜成纤维细胞中I型胶原蛋白和VEGF的水平，但未降低TGF-β1的mRNA水平。对粘连成纤维细胞进行DHA治疗可使I型胶原蛋白mRNA水平降至正常腹膜成纤维细胞的水平，与未治疗的粘连成纤维细胞相比，mRNA水平降低了35%。DHA处理的粘连成纤维细胞中VEGF mRNA水平比未处理的粘连成纤维细胞低50%。与未处理的正常腹膜成纤维细胞相比，DHA可使处理后的粘连成纤维细胞中TGF-β1 mRNA水平降至正常水平。
二十二碳六烯酸可显著降低正常腹膜和成纤维细胞中粘连相关标志物的水平。本研究为一种易于给药且可能高效的抗粘连佐剂提供了分子基础。
