采用荧光猝灭法研究氯雷他定与血清白蛋白的相互作用。
Interaction of loratadine with serum albumins studied by fluorescence quenching method.
作者信息
Zhou Bo, Qi Zu-De, Xiao Qi, Dong Jia-Xin, Zhang Ye-Zhong, Liu Yi
机构信息
Department of Chemical Biology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
出版信息
J Biochem Biophys Methods. 2007 Aug 1;70(5):743-7. doi: 10.1016/j.jbbm.2007.04.001. Epub 2007 Apr 12.
The interactions between loratadine and bovine serum albumin (BSA) and human serum albumin (HSA) were studied using tryptophan fluorescence quenching method. The fluorescence intensity of the two serum albumins could be quenched 70% at the molar ratio [loratadine]:[BSA (or HSA)]=10:1. In the linear range (0-50 micromol L(-1)) quenching constants were calculated using Stern-Volmer equation. Temperature in the range 298 K-310 K had a significant effect (p<0.05) on the two serum albumins through ANOVA analysis and t-test. Furthermore the conformation changes in the interactions were studied using FTIR spectroscopy.
采用色氨酸荧光猝灭法研究了氯雷他定与牛血清白蛋白（BSA）和人血清白蛋白（HSA）之间的相互作用。当[氯雷他定]：[BSA（或HSA）]的摩尔比为10:1时，两种血清白蛋白的荧光强度可被猝灭70%。在0至50 μmol L⁻¹的线性范围内，使用斯特恩-沃尔默方程计算猝灭常数。通过方差分析和t检验可知，298 K至310 K范围内的温度对两种血清白蛋白有显著影响（p<0.05）。此外，利用傅里叶变换红外光谱（FTIR）研究了相互作用中的构象变化。
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