Church J S, Evans D J
Commonwealth Scientific and Industrial Research Organization, Textile and Fibre Technology, PO Box 21, Belmont 3216, Australia.
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jan;69(1):256-62. doi: 10.1016/j.saa.2007.03.041. Epub 2007 Mar 31.
A spectroscopic investigation into the reaction of sodium tetrathionate with cysteine at pH 5 both at the boil and at room temperature has been carried out. The Raman and infrared spectra of the model compounds cysteine, cysteine-S-sulfonate, cysteine-S-thiosulfonate, sodium thiosulfate and sodium sulfite were also obtained and vibrations involving the sulfur atoms were analyzed in detail. These results were utilized in the interpretation of the spectra obtained from tetrathionate-cysteine reaction mixtures. The reaction supernatants were analyzed by high performance thin layer chromatography while the precipitates were analyzed gravimetrically. It was found that during the reaction, the thiol groups of cysteine are oxidised to give predominantly cysteine-S-sulfonate. Cystine was also detected but was determined gravimetrically to be a minor reaction product. No significant amounts of cysteine-S-thiosulfonate were detected. The reaction is accompanied by the formation of elemental sulfur and a small amount of sulfite. Major reaction pathways are put forth that are consistent with the experimental data.
已对连四硫酸钠与半胱氨酸在pH 5、沸腾及室温条件下的反应进行了光谱研究。还获得了模型化合物半胱氨酸、半胱氨酸-S-磺酸盐、半胱氨酸-S-硫代磺酸盐、硫代硫酸钠和亚硫酸钠的拉曼光谱和红外光谱，并对涉及硫原子的振动进行了详细分析。这些结果被用于解释从连四硫酸钠-半胱氨酸反应混合物获得的光谱。反应上清液通过高效薄层色谱法进行分析，沉淀物则采用重量分析法进行分析。结果发现，在反应过程中，半胱氨酸的巯基被氧化，主要生成半胱氨酸-S-磺酸盐。还检测到了胱氨酸，但通过重量分析法确定其为次要反应产物。未检测到大量的半胱氨酸-S-硫代磺酸盐。该反应伴随着元素硫和少量亚硫酸盐的形成。提出了与实验数据一致的主要反应途径。
