Shahjahan M, Yamada M, Tanaka H, Nakazawa A
Department of Biochemistry, Yamaguchi University School of Medicine, Japan.
Gene. 1991 Nov 15;107(2):313-7. doi: 10.1016/0378-1119(91)90332-6.
The structure of the gene (AK3) encoding bovine mitochondrial (mt) adenylate kinase isozyme 3 (AK3) has been determined. The gene spans about 46 kb and splits into five exons. The 5'-flanking region of the gene lacks typical transcriptional regulatory elements such as TATA and CAAT boxes. The G + C content of this region is high (71%), and seven GC boxes are recognized. Possible sequences responsive to transcription factors, AP-1, AP-2, Myf-6 and MyoD1, are present in this region. In the 5'-flanking region, there are sequences (mt sequence) which were found in the genes encoding other mt proteins. Transcriptional mapping analyses revealed one major mRNA start point. The promoter activity measurement in Chinese hamster ovary cells by transient expression of the cat gene connected with the 5'-flanking region, showed presence of a functional promoter.
已确定编码牛线粒体（mt）腺苷酸激酶同工酶3（AK3）的基因（AK3）的结构。该基因跨度约46 kb，分为五个外显子。该基因的5'侧翼区域缺乏典型的转录调控元件，如TATA盒和CAAT盒。该区域的G + C含量很高（71%），可识别出七个GC盒。该区域存在可能对转录因子AP-1、AP-2、Myf-6和MyoD1有反应的序列。在5'侧翼区域，存在于编码其他线粒体蛋白的基因中发现的序列（线粒体序列）。转录图谱分析揭示了一个主要的mRNA起始点。通过与5'侧翼区域相连的cat基因的瞬时表达，在中国仓鼠卵巢细胞中进行的启动子活性测量表明存在功能性启动子。
