Moorthy Guhapriya, Murali Malliga Raman, Devaraj S Niranjali
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, India.
Nutrition. 2007 May;23(5):424-33. doi: 10.1016/j.nut.2007.03.003.
Studies on lactic acid bacteria exemplify their use against various enteropathogens in vitro. Nevertheless, in vivo effects of Lactobacillus during Shigella infection have not been evaluated. The present study evaluated the effect of Lactobacillus rhamnosus and Lactobacillus acidophilus on neutrophil infiltration and lipid peroxidation during Shigella dysenteriae 1-induced diarrhea in rats.
The rats were divided into eight groups (n = 6 in each group). Induced rats received single oral dose of S. dysenteriae (12 x 10(8) colony-forming units [cfu]/mL). Treated rats received L. rhamnosus (1 x 10(7) cfu/mL) or L. acidophilus (1 x 10(7) cfu/mL) orally for 4 d, alone or in combination, followed by Shigella administration. At the end of the experimental period, animals were sacrificed and the assay of the activity of alkaline phosphatase, myeloperoxidase, and antioxidants and the estimation of lipid peroxides were performed. Activity staining of superoxide dismutase and catalase was done in addition to gelatin zymography for matrix metalloproteinase (MMP; MMP-2 and MMP-9) activity. A portion of the intestinal tissue was fixed in 10% formalin for histologic studies.
Administration of S. dysenteriae 1 alone resulted in increased levels of myeloperoxidase, lipid peroxidation, alkaline phosphatase, and the expression of MMP-2 and MMP-9 with concomitant decrease in the antioxidant levels. Pretreatment with the combination of L. rhamnosus (1 x 10(7) cfu/mL) and L. acidophilus (1 x 10(7) cfu/mL) significantly attenuated these changes when compared with the diseased group. Histologic observations were in correlation with biochemical parameters.
Lactobacillus rhamnosus plus L. acidophilus offered better protection when compared with individual treatment with these strains during Shigella infection.
关于乳酸菌的研究例证了其在体外对各种肠道病原体的作用。然而，鼠李糖乳杆菌在志贺氏菌感染期间的体内效应尚未得到评估。本研究评估了鼠李糖乳杆菌和嗜酸乳杆菌对痢疾志贺氏菌1型诱导的大鼠腹泻期间中性粒细胞浸润和脂质过氧化的影响。
将大鼠分为八组（每组n = 6）。诱导组大鼠单次口服痢疾志贺氏菌（12×10⁸ 菌落形成单位[cfu]/mL）。治疗组大鼠单独或联合口服鼠李糖乳杆菌（1×10⁷ cfu/mL）或嗜酸乳杆菌（1×10⁷ cfu/mL）4天，随后给予志贺氏菌。在实验期结束时，处死动物并进行碱性磷酸酶、髓过氧化物酶和抗氧化剂活性测定以及脂质过氧化物的评估。除了对基质金属蛋白酶（MMP；MMP - 2和MMP - 9）活性进行明胶酶谱分析外，还进行了超氧化物歧化酶和过氧化氢酶的活性染色。取一部分肠道组织固定在10%福尔马林中用于组织学研究。
单独给予痢疾志贺氏菌1型导致髓过氧化物酶、脂质过氧化、碱性磷酸酶水平升高以及MMP - 2和MMP - 9表达增加，同时抗氧化剂水平降低。与患病组相比，用鼠李糖乳杆菌（1×10⁷ cfu/mL）和嗜酸乳杆菌（1×10⁷ cfu/mL）联合预处理可显著减轻这些变化。组织学观察结果与生化参数相关。
在志贺氏菌感染期间，与单独使用这些菌株治疗相比，鼠李糖乳杆菌加嗜酸乳杆菌提供了更好的保护。
